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2321-07-5

中文名稱 熒光素
英文名稱 Fluorescein
CAS 2321-07-5
EINECS 編號 219-031-8
分子式 C20H12O5
MDL 編號 MFCD00005050
分子量 332.31
MOL 文件 2321-07-5.mol
更新日期 2024/12/19 14:33:05
2321-07-5 結(jié)構(gòu)式 2321-07-5 結(jié)構(gòu)式

基本信息

中文別名
2-(6-羥基-3-氧代-3H-呫噸-9-基)苯甲酸
螢光橙黃
螢光紅
螢光黃
螢光素
熒光酯
熒光橙紅
熒光黃
熒光素,水溶
黃 7 [CI 45350:1]
熒光素99%
螢光黃
二酚酞
熒光素
2-(6-羥基-3-氧代-3H-噸-9-基)苯甲酸
熒光素, 90+%
英文別名
ACID YELLOW 73
CI 45350
CI 45350.1
CI 45350:1
CI NO 45350
D AND C YELLOW NO 7
FLUORESCEIN
FLUORESCEIN, ALCOHOL SOLUBLE
RESORCINOL PHTHALEIN
S NO 880
SOLVENT YELLOW 94
11712yellow
2-(6-hydroxy-3-oxo-3h-xanthen-9-yl)-benzoicaci
3,6-dihydroxyspiro(xanthene-9,3’-phthalide)
3,6-fluorandiol
3’,4’-dehydroxyfluoran
3’,6’-dihydroxy-fluora
3’,6’-dihydroxyfluoran
3’,6’-dihydroxyspiro(isobenzofuran-1(3h),9’(9h)-xanthen)-3-one
3’,6’-fluorandiol
所屬類別
生物化工:生物染料

物理化學(xué)性質(zhì)

外觀性狀橙黃或淺橙紅色結(jié)晶粉末。熔點314-316℃(分解)。溶于熱乙醇、冰醋酸、堿溶液,呈綠色螢光,不溶于水、苯、氯仿和乙醚。
熔點320 °C(lit.)
沸點429.44°C (rough estimate)
密度1.2739 (rough estimate)
折射率1.5000 (estimate)
儲存條件−20°C
溶解度不溶于水、乙醚、苯、氯仿;溶于乙醇、甲醇、丙酮、乙酸乙酯、N,N-二甲基甲酰胺
色指數(shù)45350
酸度系數(shù)(pKa)2.2, 4.4, 6.7(at 25℃)
形態(tài)粉末
顏色紅色到橙色
酸堿指示劑變色ph值范圍Pink uorescence (4.0) to green uorescence (6.0)
水溶解性insoluble
最大波長(λmax)493.5nm, 496nm, 460nm, 515nm
Merck14,4159
BRN94324
穩(wěn)定性穩(wěn)定的。易燃。與強(qiáng)氧化劑不相容。
生物領(lǐng)域應(yīng)用Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity
主要應(yīng)用Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer
LogP2.980 (est)
CAS 數(shù)據(jù)庫2321-07-5(CAS DataBase Reference)
EPA化學(xué)物質(zhì)信息Fluorescein (2321-07-5)

安全數(shù)據(jù)

危險性符號(GHS)GHS hazard pictograms
GHS07
警示詞警告
危險性描述H319
防范說明P305+P351+P338
危險品標(biāo)志Xi
危險類別碼R43-R36
WGK Germany3
RTECS號LM5075000
TSCAYes
海關(guān)編碼32042000
毒害物質(zhì)數(shù)據(jù)2321-07-5(Hazardous Substances Data)

應(yīng)用領(lǐng)域

用途一
汞溴紅的中間體。螢光光度分析試劑,測定氯、溴、碘、硫離子;氧化-還原指示試劑;生物染色劑。
用途二
熒光光度分析硫離子,試驗溴,滴定氯、溴和碘。吸附指示劑,氧化還原指示劑。

制備方法

方法一
用鄰苯二甲酸酐與間苯二酚在硫酸參與下反應(yīng)制得。

化學(xué)品安全說明書(MSDS)

熒光素價格(試劑級)
報價日期產(chǎn)品編號產(chǎn)品名稱CAS號包裝價格
2024/11/08L13251熒光素, 90+%
Fluorescein
2321-07-5100g635元
2024/11/08L13251熒光素, 90+%
Fluorescein
2321-07-5500g1549元
2024/11/0811924熒光素
Fluorescein, pure
2321-07-5100g511元

常見問題列表

生物活性
Fluorescein (Resorcinol phthalein) 是一種合成的有機(jī)光敏染料化合物。Fluorescein 是熒光示蹤劑,可以應(yīng)用于醫(yī)學(xué)和生物學(xué)以及腫瘤感染的組織。
體外研究

Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays.
The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.

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