17086-76-9
17086-76-9 結(jié)構(gòu)式
基本信息
杯莧甾酮
杯莧甾酮(標(biāo)準(zhǔn)品)
杯莧甾酮(杯覓甾酮)
杯莧甾酮, 來源于川牛膝
CYASTERONE 杯莧甾酮
CYASTERONE
(22r,24s,25s,28r)-gamma-lacton
Cyasterone, 98%, from Cyathula officinalis K.C. Kuan
2-beta,3-beta,14,20,22,28-hexahydroxy-6-oxo-5-beta-stigmast-7-en-26-oicaci
(22R,24S,25S,28R)-2β,3β,14,20,22,28-Hexahydroxy-6-oxo(26-13C)-5β-stigmasta-7-ene-26-oic acid γ-lactone
7,(5BETA)-CHOLESTEN-26-CARBOXYLIC ACID-24BETA-(1-HYDROXYETHYL)-2BETA, 3BETA, 14ALPHA,20BETA, 22R-PENTOL-6-ONE GAMMA-LACTONE
(3S,4S,5R)-4-[(2R,3R)-2,3-Dihydroxy-3-[(2S,3R,5R,9R,10R,13R,14S,17S)-2,3,14-trihydroxy-10,13-dimethyl-6-oxo-2,3,4,5,9,11,12,15,16,17-decahydro-1H-cyclopenta[a]phenanthren-17-yl]butyl]-3,5-dimethyloxolan-2-one
物理化學(xué)性質(zhì)
應(yīng)用領(lǐng)域
藥理藥效:養(yǎng)血舒筋, 祛風(fēng)除濕。
常見問題列表
IC50: EGFR
Cyasterone exerts cytotoxicity on multiple cell lines: HeLa (IC 50 =77.24 μg/ml); HepG-2 (IC 50 =52.03 μg/ml); MCF-7 (IC 50 =82.07 μg/ml) and MCF-7 (IC 50 =82.07 μg/ml). And It has none cytotoxicity (IC 50 >400 μg/ml) on 3 types of carcinoma (HT-29, Caco-2, T47D) and 1 type of normal (NIH 3T3) cell lines. Cyasterone (0-60 μg/ml; 48 hours) causes a significantly decreasing of the cell proliferation. It inhibits cell growth as a dose-dependent manner, exhibits IC 50 values of 38.50 μg/ml and 32.96 μg/ml, respectively. Cyasterone (0-60 μg/ml; 24 hours) decreases p-EGFR, p-MEK, and p-mTOR as a dose-dependent manner in A549 cells, it affects the MAPK signaling pathway activities.
Cell Viability Assay
| Cell Line: | A549 cells and MGC823 cells |
| Concentration: | 0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml |
| Incubation Time: | 24 hours |
| Result: | Inhibited cell proliferation as a dose-dependent manner. |
Western Blot Analysis
| Cell Line: | A549 cells |
| Concentration: | 0 μg/ml, 20 μg/ml, 40 μg/ml, 60 μg/ml |
| Incubation Time: | 24 hours |
| Result: | Surpressed p-EGFR, p-MEK, and p-mTOR expression. |
Cyasterone (intraperitoneal injection; 5 mg/kg, 10 mg/kg and 15 mg/kg; 21 days) has anti-proliferation effect in vivo and inhibits MGC823 cells xenografted tumor growth in vivo with few changes in body weights.
| Animal Model: | MGC823 xenograft tumor in BALB/C-nu mice |
| Dosage: | 5 mg/kg, 10 mg/kg and 15 mg/kg |
| Administration: | Intraperitoneal injection |
| Result: | Inhibited MGC823 xenograft tumor growth in vivo. |