115144-35-9
基本信息
BEETLE LUCIFERIN, POTASSIUM SALT
D-(-)-2-[6'-HYDROXY-2'-BENZOTHIAZOLYL]DELTA-THIAZOLINE-4-CARBOXYLIC ACID POTASSIUM SALT
D-LUCIFERIN FIREFLY, POTASSIUM SALT
D-LUCIFERIN, MONOPOTASSIUM SALT
D-LUCIFERIN POTASSIUM SALT
D -LUCIFERIN SYNTHETIC POTASSIUM SALT
D-Luciferenpotassiumsalt
(S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylicacid,potassiumsalt
(D)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid, Firefly, potassium salt
D-Luciferin*K
D-LUCIFERINPOTASSIUMSALT,99%
物理化學(xué)性質(zhì)
DMSO:7.5(Max Conc. mg/mL);23.55(Max Conc. mM)
Ethanol:0.25(Max Conc. mg/mL);0.79(Max Conc. mM)
PBS (pH 7.2):10.0(Max Conc. mg/mL);0.31(Max Conc. mM)
Water:32.0(Max Conc. mg/mL);100.5(Max Conc. mM)
應(yīng)用領(lǐng)域
常見(jiàn)問(wèn)題列表
D-熒光素鉀鹽是熒光素酶的水溶性底物,存在于多種發(fā)光生物體中。在A(yíng)TP和熒光素酶的催化作用下,D-熒光素鉀被氧化,產(chǎn)生藍(lán)綠色的光(560nm),當(dāng)?shù)孜镞^(guò)量時(shí),產(chǎn)生的光量子數(shù)與熒光素酶的濃度呈正相關(guān)。編碼熒光素酶的Luc基因是植物、哺乳動(dòng)物細(xì)胞的常用報(bào)告基因。由于沒(méi)有背景干擾,因此可以很容易地檢測(cè)出低至0.02 pg 水平的熒光素酶。
D-熒光素鉀鹽是一類(lèi)在生物體中發(fā)現(xiàn)的能引起生物發(fā)光的雜環(huán)化合物,如螢火蟲(chóng)。在A(yíng)TP存在下,螢光素酶將其氧化脫羧后會(huì)發(fā)光?;瘜W(xué)研究中可用于熒光素酶的基板。
D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly
Photinus pyralis
, the enzyme more frequently used in techniques of in vitro and optical imaging.
D-Luciferin exhibits a decrease in the measured K
m
in PC3M-Luc cell lysates with a K
m
of 34 μM.
Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20?min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection.
Inject with 10 μL of D-luciferin (intraperitoneally or intravenously) stock solution per gram of body weight: normally ~200 μL for a 20 g mouse for a standard 150 mg/kg injection.
Thaw D-Luciferin (either Potassium or Sodium Salt) at room temperature and dissolve in dPBS (no calcium or magnesium) to a final concentration of 15 mg/mL. Pre-wet a 0.22 μm filter by drawing through 5-10 mL of sterile H
2
O and discard water. Sterilize the D-Luciferin solution through the prepared 0.22 μm syringe filter.