2,6-Dichloropurine-2'-deoxyriboside synthesis
- Product Name:2,6-Dichloropurine-2'-deoxyriboside
- CAS Number:37390-66-2
- Molecular formula:C10H10Cl2N4O3
- Molecular Weight:305.12
5451-40-1
503 suppliers
$10.00/1g
50-89-5
634 suppliers
$9.00/1g
65-71-4
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$5.00/5g
37390-66-2
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Yield:37390-66-2 36 mg
Reaction Conditions:
with potassium phosphate at 40; pH=7.5; for 1 h;Equilibrium constant;Enzymatic reaction;
Steps:
4.3. Enzymatic Production of Dihalogenated Purine Nucleosides
General procedure: To produce dihalogenated nucleoside analogues, base concentrations of 5 mM and sugar donorconcentrations of 22 to 30.5 mM were applied in a reaction volume of 50 mL. Thermostable pyrimidineand purine nucleoside phosphorylase PyNP 02 and PNP 02 were added in a concentration of 0.1 mgmL1 each. Enzymatic reactions were performed in 0.5 mM potassium phosphate buer at pH 7.5using a reaction temperature of 40 °C. Reactions were stirred at 100 rpm. Regular samples were takento monitor enzymatic reactions by HPLC as described above. The reactions were stopped by enzyme removal after 2 h and 6 h for deoxyriboside and ribosidenucleosides, respectively. Protein was removed by filtration using centrifugal filter units with a cut-oof 30 kDa (Millipore, Darmstadt, Germany) at room temperature. Filtrate was stored at 4 °C untilpurification by semi-preparative HPLC.Analytical HPLC was performed to determine product quantity after each step. Product loss perstep was determined based on the product quantity of step 1 (e.g., after enzymatic synthesis) and theproduct quantity of the following step (e.g., after protein depletion and HPLC).
References:
Giessmann, Robert T.;Kaspar, Felix;Klare, Hendrik F. T.;Kurreck, Jens;Neubauer, Peter;Paulick, Katharina;R?hrs, Viola;Wagner, Anke;Westarp, Sarah;Yehia, Heba [Molecules,2020,vol. 25,# 4]
951-78-0
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5451-40-1
503 suppliers
$10.00/1g
37390-66-2
30 suppliers
inquiry