ChemicalBook CAS DataBase List 2'-Deoxyuridine

2'-Deoxyuridine synthesis

11synthesis methods

Product Name:2'-Deoxyuridine
CAS Number:951-78-0
Molecular formula:C9H12N2O5
Molecular Weight:228.2

951-77-9 Synthesis

951-77-9
415 suppliers
$5.00/100mg

951-78-0
486 suppliers
$5.00/100mg

Yield:951-78-0 99%

Reaction Conditions:

with cytidine deaminase enzyme in aq. phosphate buffer at 37; pH=7; for 0.0833333 h;Enzymatic reaction;

Steps:

3 Comparative Example 3: Deamination of 2'-deoxycytidine to 2'-deoxyuridine

Comparative Example 3: Deamination of 2'-deoxycytidine to 2'-deoxyuridine A 100 mM solution of 2'-deoxycytidine (495 μΙ_) in 100 mM phosphate buffer at pH 7 was mixed with 50 μΙ_ of cytidine deaminase enzyme solution containing >300 AU in phosphate buffer. The reaction was performed at 37°C during 5 minutes and stopped with HCI. Then, the crude reaction was filtered through a 10 KDa membrane, and a portion was diluted and analyzed by HPLC-UV-DAD. Product identification was performed by comparison to a standard sample. 2'-Deoxyuridine was obtained in quantitative yield (>99%).

References:

WO2016/146808,2016,A1 Location in patent:Page/Page column 29