成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

ChemicalBook >> journal list >> General physiology and biophysics >>article
General physiology and biophysics

General physiology and biophysics

IF: 1.3
Download PDF

Effect of amphiphilic surfactant LDAO on the solubilization of DOPC vesicles and on the activity of Ca(2+)-ATPase reconstituted in DOPC vesicles.

Published:1 December 2007 DOI: PMID: 18281747
J Karlovská, F Devínsky, P Balgavy

Abstract

Solubilization of large unilamellar 1,2-dioleoylphosphatidylcholine (DOPC) vesicles by N-dodecyl-N,N-dimethylamine-N-oxide (LDAO) was studied using turbidimetry. From turbidity data, the LDAO partition coefficient between the aqueous phase and DOPC bilayers was obtained. Using this partition coefficient, the LDAO:DOPC molar ratio in the bilayer was calculated and effects of LDAO on the bilayer stability, bilayer thickness and on the phosphohydrolase activity of sarcoplasmic reticulum Ca(2+) transporting ATPase (SERCA) reconstituted into DOPC were compared at the same LDAO:DOPC molar ratios in the bilayer. The sequence "bilayers in vesicles - bilayer fragments (flat mixed micelles) - tubular mixed micelles - globular mixed micelles" was suggested for the solubilization mechanism of DOPC vesicles from the combined turbidimetric and small-angle neutron scattering (SANS) results. The effective molecular packing parameter delta = 0.5, corresponding to the mixed bilayer - mixed tubular micelle transition, was calculated from fragmental DOPC and LDAO volumes at the molar ratio LDAO:DOPC = 2.00 in bilayers, in the middle of transition region observed earlier experimentally by small-angle neutron scattering (SANS). The bilayer thickness decrease induced by LDAO in DOPC observed by SANS did not result in the SERCA phosphohydrolase activity decrease and this indicates that some other factors compensated this bilayer effect of LDAO. The ATPase activity decrease at higher LDAO concentrations was caused by the bilayer deformation. This deformation resulted in the formation of non-bilayer aggregates in LDAO+DOPC system.

Substances (5)

Related products
Procduct Name CAS Molecular Formula Supplier Price
LDAO 1643-20-5 C14H31NO 454 suppliers $19.00-$2330.00
1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE 4235-95-4 C44H84NO8P 236 suppliers $34.00-$685.00
ADENOSINE 5'-TRIPHOSPHATASE 9000-83-3 C10H16N5O13P3 116 suppliers $201.00-$749.00
Human calcium magnesium ATPase (CA~(2+)-MG~(2+)-ATPASE) ELISA kit 6 suppliers Inquiry
Serca 1 suppliers Inquiry

Similar articles

IF:5.7

Inhibitory effect of the peptide epitalon on the development of spontaneous mammary tumors in HER-2/neu transgenic mice

International Journal of Cancer Vladimir N. Anisimov, Vladimir KH. Khavinson,etc Published: 25 July 2002
IF:2.78

The The Effect of Alcohols and of Alcohols and Salts on the Critical Micelle Concentration of Dodecylammonium Chloride.

The Journal of Physical Chemistry S. H. Herzfeld, M. L. Corrin,etc Published: 1 February 1950
IF:4.8

The Benefits of Olive Oil for Skin Health: Study on the Effect of Hydroxytyrosol, Tyrosol, and Oleocanthal on Human Fibroblasts.

Nutrients Anabel González-Acedo, Javier Ramos-Torrecillas,etc Published: 25 April 2023