成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

ChemicalBook >> journal list >> Oncology reports >>article
Oncology reports

Oncology reports

IF: 3.79
Download PDF

Regulation of the β?catenin/LEF?1 pathway by the siRNA knockdown of RUVBL1 expression inhibits breast cancer cell proliferation, migration and invasion

Published:1 February 2025 DOI: 10.3892/or.2024.8855 PMID: 39670302
Xin Zhang,?Dingyuan Cui,?Wei Sun,?Guangfei Yang,?Wen Wang,?Chengrong Mi

Abstract

RUVBL1 is a protein characterized by its DNA?dependent ATPase activity and DNA deconjugating enzyme function. It is a member of the ATPase (AAA+) protein family associated with various cellular processes. Available research confirms that the expression of RUVBL1 is upregulated in breast cancer (BRCA) cell lines; however, the mechanisms underlying its functional role in BRCA remain unclear. The β?catenin/lymphoid enhancer factor?1 (LEF?1) pathway plays a crucial role in the occurrence and development of BRCA. The aim of the present study was to investigate whether RUVBL1 regulates the proliferation, migration and invasion of BRCA cells by participating in the β?catenin/LEF?1 signaling pathway. Reverse transcription?quantitative PCR (RT?qPCR) and western blot analysis were employed to compare the RUVBL1 expression levels between normal mammary epithelial cells (MCF?10a) and BRCA cell lines (MDA?MB?231 and MCF?7). Scratch, Cell Counting Kit?8 and Transwell assays were utilized to assess the effects of RUVBL1 knockdown on the proliferation, migration and invasion of BRCA cells. Following the downregulation of RUVBL1 expression in vitro, western blot analysis and RT?qPCR were conducted to investigate its role in regulating the β?catenin/LEF?1 pathway. The aforementioned experiments proved that the knockdown of RUVBL1 expression inhibited BRCA cell proliferative, migratory and invasive capabilities, modulating the β?catenin/LEF?1 pathway. Collectively, the findings of the present study provide preliminarily confirmation that RUVBL1 participates in the molecular mechanisms of the β?catenin signaling pathway, which may provide a novel target for BRCA treatment.

Substances (1)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Crystal Violet 548-62-9 C25H30ClN3 759 suppliers $5.00-$2070.00

Similar articles

IF:4.5

FMNL3 Promotes Migration and Invasion of Breast Cancer Cells via Inhibiting Rad23B‐Induced Ubiquitination of Twist1

Neural Regeneration Research Binggong Zhao, Dong‐Man Ye,etc Published: 25 November 2024
IF:3.8

Salidroside inhibits the growth of human breast cancer in vitro and in vivo.

Neural Regeneration Research Gang Zhao, Aiping Shi,etc Published: 1 May 2015