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Journal of Chromatography A

Journal of Chromatography A

IF: 3.79
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Microchip capillary electrophoresis-mass spectrometry for high-throughput simultaneous analysis of B-complex vitamins

Published:6 December 2024 DOI: 10.1016/j.chroma.2024.465589 PMID: 39662335
Liu Yang,?Shuang Wang,?Xiaolong Li,?Weimin Wang,?Fuxing Xu,?Chuan-Fan Ding

Abstract

B-complex vitamins are essential micronutrients that maintaining health, and provide (individually/simultaneously) many important biological actions in organism. Therefore, sensitive, reliable analytical method to determine B-complex vitamins simultaneously in actual samples is significant. Conventional analytical methods for vitamins analysis are usually labor-intensive, time-consuming and mostly do not allow the simultaneous determination. Few papers have been published on capillary electrophoresis-mass spectrometry (CE-MS) analysis of individual vitamin B or B-complex vitamins. Microchip capillary electrophoresis (ME) offers less consumption of background electrolyte and reagents than CE, and fast separation as having several channels for high-throughput. ME based on ZipChip integrates sample injection, electrophoretic separation, and ESI functions into one platform, herein, for the first time, in this paper, the ME-MS based on ZipChip platform was investigated and demonstrated preliminarily a significant improvement over existing techniques. Because: the ME-MS method has a linear response with excellent precision for replicate injections and LODs lower than 4 ppb; The method is simple and fast (<3 min per sample), using a small amount of sample (~5 nL) with low reagent consumption, making it suitable for high-throughput applications; The inter/intraday reproducibility (RSD %) is less than 3 %, which is important for clinic application.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Ethylenediaminetetraacetic acid 60-00-4 C10H16N2O8 1166 suppliers $10.00-$6397.62
Trichloroacetic acid 76-03-9 C2HCl3O2 479 suppliers $14.00-$4601.00