成人免费xx,国产又黄又湿又刺激不卡网站,成人性视频app菠萝网站,色天天天天

ChemicalBook >> journal list >> Poultry Science >>article
Poultry Science

Poultry Science

IF: 3.79
Download PDF

miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken

Published:25 November 2024 DOI: 10.1016/j.psj.2024.104583
Qingqing Geng, Cai Hu, Yani Zhang

Abstract

Spermatogonial stem cells (SSCs) have vast application prospects in livestock and poultry production, genetic engineering, and medical research. However, the scarcity of SSCs and the complexity of their development limit the elucidation and verification of the mechanism of SSCs in vitro. Although miRNAs have been identified as critical players in germ cell development, upstream regulatory mechanisms by which miRNAs regulate SSCs formation are rarely reported. In this study, miR-1458, which was differentially expressed during SSCs formation, was selected by transcriptomic sequencing. We found that miR-1458, inhibited in an in vitro SSCs induction model,
significantly upregulated the expression of germline marker genes (Cvh and integrin β1). Further analysis using Immunofluorescence and Flow Cytometry confirmed that miR-1458 inhibition promotes the formation of spermatogonial stem-like cells (SSCLCs). Immunohistochemical significantly increased the number of SSCs in the testis in vivo. However, significant upregulation of miR-1458 showed opposite results. High-throughput sequencing results showed that miR-1458 interacted with TBX6, one of the target genes of miR-1458, involved in affecting cell differentiation, and dual-luciferase reporter vectors confirmed the targeting relationship between the two. TBX6 overexpression and knockdown in vitro and in vivo have validated its function in SSCs formation. We found that overexpression of TBX6 promoted SSCs formation. Additionally, we identified Vitamin B6, a key metabolite affecting SSCs formation, as an upstream regulator of miR-1458 expression. The results showed that low concentrations of Vitamin B6 led to low expression of miR-1458 by decreasing histone demethylation levels. Overall, our findings suggest that miR-1458 is involved in SSCs formation, which is inhibited by low concentrations of Vitamin B6 and subsequently regulates the formation of SSCs by targeting TBX6, an essential gene involved in embryonic stem cell differentiation. Our study demonstrates the critical role of the Vitamin B6-miR-1458-TBX6 regulatory axis in spermatogonial stem cell formation in Rugao Yellow Chicken, providing new insights into the regulatory mechanisms by which miRNAs affect SSCs formation. It should be noted that most of the germline findings related to miRNAs were obtained by in vitro studies, and in vivo studies are needed to validate our results for clinical applications.

Substances (2)

Materials
Procduct Name CAS Molecular Formula Supplier Price
Triton X-100 9002-93-1 (C2H4O)nC14H22O 532 suppliers $18.00-$610.00
4',6-Diamidino-2-phenylindole dihydrochloride 28718-90-3 C16H16ClN5 295 suppliers $14.10-$4800.00

Similar articles

IF:5.4

Dietary Intake of Vitamin B12 is Better for Restoring a Low B12 Status Than a Daily High-Dose Vitamin Pill: An Experimental Study in Rats.

ACS Applied Energy Materials Eva Greibe, Ole Nymark,etc Published: 15 August 2018
IF:3

MiR-23a-3p targets PTEN as a novel anti-ferroptosis regulator in Fuchs endothelial corneal dystrophy

Experimental eye research Miaomiao Chi, Yaning Zhao,etc Published: 22 November 2024