產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價格
AF2647
STAT5b Rabbit Monoclonal Antibody
50μl
618.00元
Rabbit
WB, IP, IF, IHC, ICC
H, M, R
90.0kDa
???????WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin
Immunoprecipitation Assay.
???????H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
???????配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
???????建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000-1:2,000
1:10-1:50
1:100-1:500
1:50-1:200
1:100-1:500
-
-
-
抗體詳細信息如下:
Name
STAT5b Rabbit Monoclonal Antibody
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Purification
Affinity purification
Immunogen
Recombinant protein
Gene ID
6777(Human); 20851(Mouse); 25126(Rat)
SwissProt
P51692(Human); P42232(Mouse); P52632(Rat)
Category
Immunology/Inflamation
Background
Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96% homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phos-phorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4-mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retin-oic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.
包裝清單:
AF2647
STAT5b Rabbit Monoclonal Antibody
50μl
保存條件:
???????STAT5b Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
???????如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
???????回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
???????提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(ICC)等適當用途。如果希望獲得的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
???????本產(chǎn)品僅限于專業(yè)人員的科學研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
???????為了您的安全和健康，請穿實驗服并戴一次性手套操作。