背景資料

p53， a 53 kDa protein， binds to a DNA consensus sequence， the p53 response element， and regulates normal cell cycle events by activating transcription of genes involved either in progression through the cell cycle， or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells， principally due to the increased half-life （4 hrs） compared to that of wild-type （20 min）. p53 localizes in the nucleus， but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression， but may not always be necessary for apoptosis.

應(yīng)用類型

Western blotting （0.5-4 ug/ml）， immunoprecipitation （4-8 ug/ml）， Immunohistochemistry （4-8 ug/ml）， immunocytochemistry （4-8 ug/ml）. However， the optimal conditions should be determined individually.

免疫原

Synthetic peptide corresponding to residue surrounding Ser20 of human p53

來(lái)源宿主

小鼠

反應(yīng)性

經(jīng)檢測(cè)驗(yàn)證，可與人、小鼠、大鼠種屬來(lái)源的p53蛋白發(fā)生免疫反應(yīng).

保存建議

提供的p53抗體產(chǎn)品為100 ug 無(wú)色溶液，其濃度為0.5 mg/ml，溶解于包含有1 mg/ml BSA和1.5 mM 疊氮鈉和50%甘油.的PBS緩沖液中.請(qǐng)將該p53抗體產(chǎn)品置于-20 ℃冷凍保存，建議分裝使用以避免反復(fù)凍融.