Recombinant Carboxypeptidase B

Catalog Number:RCPB01

CAS: 9025-24-5
EC: 3.4.17.2

Storage temperature: 2-8℃

Source: Recombinant carboxypeptidase B is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant carboxypeptidase B is identical to rat carboxypeptidase B

1.DESCRIPTION

Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, pI is 6.0 and the optimum pH is 7.5-9.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. YaxinBio has cloned rat carboxypeptidase B gene into  E.coli for expression with equivalent properties compared to animal original carboxypeptidase B .Recombinant carboxypeptidase B can replace native carboxypeptidase B for using in a variety of biotechnological processes.

2.MAIN FEATURES

UNIT DEFINITION：One unit of carboxypeptidase B activity hydrolyzes one micromole ofhippuryl-L-arginine per minute at 25℃, pH 7.65.

3.RECOMMEND USAGE 

Prepare 1-10mg/ml recombinant carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.5-9.0. 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.After dissolved with sterile water or 25mM Tris-HCl, it should be stored under -20℃. 

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

①　Animal origin free: Recombinant carboxypeptidase B is no exogenous virus contamination,and any animal origin material is not used in the production process.

②　Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.

③　High purity: Higher specific activity.No other contaminating proteases such as chymotrypsin and carboxypeptidase A.Host protein residues is less than the limits of biological products.

④　Lyophilized powder: The product is lyophilized powder and is convenient to store and transport.

⑤　Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.

⑥　Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.PRODUCT USAGE

The production of recombinant insulin and its analogues;

the quality test of recombinant antibodies;

the determination of C-terminal amino acids of proteins;

the production of other recombinant polypeptides;

and the enzymatic synthesis of certain specific compounds.

7.RELATED PRODUCTS

Recombinant trypsin

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase