磷酸氯喹|T0194|TargetMol

價(jià)格	￥298	￥418	￥656
包裝	50mg	100mg	200mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-09-23

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產(chǎn)品詳情

中文名稱:磷酸氯喹	英文名稱:Chloroquine phosphate
CAS:50-63-5	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: ≥95%	產(chǎn)品類別: 抑制劑
貨號(hào): T0194

Product Introduction

Bioactivity

名稱	Chloroquine phosphate
描述	Chloroquine phosphate (Aralen phosphate) is an aminoquinoline antimalarial and also is widely used as an autophagy inhibitor. Chloroquine also is an inhibitor of toll-like receptors (TLRs).
細(xì)胞實(shí)驗(yàn)	The cells are cultured in 6-well plates with normal culture medium in the presence of vehicle or 25 or 50 μM chloroquine, until near confluency, after which they are rinsed with sterile phosphate-buffered saline (PBS) and cultured further for the indicated times in serum-free culture medium. At the desired time-points, the culture medium is discarded and the cells are quickly harvested in lysis buffer and clarified by centrifugation. Subsequent to boiling the supernatants in reducing sodium dodecyl sulfate (SDS) sample buffer, equal amounts of protein (100 μg) are loaded per lane and the samples are electrophoresed into 10 or 4-20% gradient polyacrylamide SDS gels, then transferred to a nitrocellulose membrane. To detect TLR9, the blots were incubated overnight at 4°C with anti-TLR9 antibodies, diluted 1:500 in Tris-buffered saline with 0.1% (v/v) Tween-20 (TBST). Equal loading is confirmed with polyclonal rabbit anti-actin. Secondary detection is performed with horseradish peroxidase-linked secondary antibodies. The protein bands are visualized by chemiluminescence using an ECL kit [2].
動(dòng)物實(shí)驗(yàn)	Control and TLR9 siRNA MDA-MB-231 cells (5×105 cells in 100 μL) are inoculated into the mammary fat pads of four-week-old, immune-deficient mice (athymic nude/nu Foxn1). Treatments are started seven days after tumor cell inoculation. The mice are treated daily either with intraperitoneal (i.p.) chloroquine (80 mg/kg) or vehicle (PBS). The animals are monitored daily for clinical signs. Tumor measurements are performed twice a week and tumor volume is calculated according to the formula V=(π/6) (d1×d2)3/2, where d1 and d2 are perpendicular tumor diameters. The tumors are allowed to grow for 22 days, at which point the mice are sacrificed and the tumors are dissected for a final measurement. Throughout the experiments, the animals are maintained under controlled pathogen-free environmental conditions (20-21°C, 30-60% relative humidity and a 12-h lighting cycle). The mice are fed with small-animal food pellets and supplied with sterile water ad libitum [2].
體外活性	Chloroquine （20 μM）可抑制 IL-12p70 的釋放，并降低活化的人單核細(xì)胞衍生的朗格漢斯樣細(xì)胞（MoLC）的 Th1 誘導(dǎo)能力。Chloroquine （20 μM）可增強(qiáng) IL-1 誘導(dǎo)的 MoLC IL-23 分泌，并隨后增加啟動(dòng)的 CD4+ T 細(xì)胞的 IL-17A 釋放[1]。Chloroquine （25 μM）可抑制親代 MDA-MB-231 細(xì)胞在常氧和缺氧情況下的 MMP-9 mRNA 表達(dá)。Chloroquine 對(duì) MMP-2、MMP-9 和 MMP-13 mRNA 的表達(dá)具有細(xì)胞、劑量和缺氧依賴性影響[2]。使用Chloroquine 抑制 TLR7 和 TLR9 可顯著減少體外 HuH7 細(xì)胞的增殖[3]。
體內(nèi)活性	在正位小鼠模型中，Chloroquine （80 mg/kg，靜脈注射）不能阻止 TLR9 表達(dá)水平高或低的三陰性 MDA-MB-231 細(xì)胞的生長(zhǎng)[2]。使用Chloroquine 抑制 TLR7 和 TLR9 可顯著抑制小鼠異種移植模型中腫瘤的生長(zhǎng)。Chloroquine 也能顯著抑制 DEN/NMOR 大鼠模型中 HCC 的發(fā)展 [3]。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : Insoluble H2O : 10 mM
關(guān)鍵字	inhibit \| Chingamin Phosphate \| COVID-19 \| Chloroquine phosphate \| Chloroquine \| Autophagy \| Inhibitor \| rheumatoid \| immune-modulating \| Toll-like Receptor (TLR) \| Antibiotic \| HIV \| SARS coronavirus \| Chloroquine Phosphate \| infection \| Parasite \| Human immunodeficiency virus \| arthritis \| Chloroquine Diphosphate \| malaria \| inflammatory \| SARS-CoV \| Aralen Phosphate
相關(guān)庫(kù)	抑制劑庫(kù) \| 經(jīng)典已知活性庫(kù) \| 已知活性化合物庫(kù) \| 生物堿類天然產(chǎn)物庫(kù) \| 抗癌天然產(chǎn)物庫(kù) \| 天然產(chǎn)物庫(kù) \| 抗病毒庫(kù) \| 膜蛋白靶向化合物庫(kù) \| 抗寄生蟲(chóng)天然產(chǎn)物庫(kù) \| 抗癌藥物庫(kù)

關(guān)鍵字： Chloroquine diphosphate|||Aralen phosphate|||Chingamin phosphate|||磷酸氯喹|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過(guò)在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過(guò)五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

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公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
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公司地址：上海市閘北區(qū)江場(chǎng)三路28號(hào)4樓

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