IRDye 650 NHS Ester

IR 650 NHS酯（IRDye? 650 NHS酯類似物）是一種水溶性，明亮，遠(yuǎn)紅熒光染料，光譜上類似于用于流式細(xì)胞術(shù)，顯微鏡和其他應(yīng)用的其他650染料。IR 650 共軛物適合 633 nm 或 647 nm 激光線。與其他熒光團(tuán)相比，使用長(zhǎng)波長(zhǎng)染料（如Cy5?染料或Alexa Fluor? 647染料）的優(yōu)勢(shì)是生物標(biāo)本在該光譜區(qū)域中的自發(fā)熒光較低。

NHS酯（或琥珀酰亞胺酯）是**常用的胺反應(yīng)基團(tuán)，用于標(biāo)記蛋白質(zhì)伯胺（Lys），胺修飾寡核苷酸和其他含胺分子。蛋白質(zhì)可以用IR 650 NHS酯以高摩爾比標(biāo)記，而不會(huì)顯著的自熄滅，從而導(dǎo)致更亮的偶聯(lián)物和更靈敏的檢測(cè)。IR 650偶聯(lián)物可用于檢測(cè)低豐度靶標(biāo)。

　　ReactivityPrimary amine

　　Abs/Em Maxima651/668 nm

　　Extinction coefficient230,000 cm-1M-1

　　SolubilityWater, DMSO, DMF

　　Spectrally similar dyesCy5, Alexa Fluor? 647, DyLight? 649,

　　Molecular weight1032.18

　　Storage conditions-20C

　　Shipping conditionsAmbient temperature

IRDye 650 Maleimide

IR 650 Maleimide (IRDye? 650 Maleimide analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5? Dye or Alexa Fluor? 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

IRDye 650 Carboxylic acid

IR 650 Carboxylic acid (IRDye? 650 Carboxylic acid analog) is a water-soluble, bright, far-red-fluorescent dye and is spectrally similar to other 650 dyes that are used for flow cytometry, microscopy and other applications. IR 650 conjugates ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5? Dye or Alexa Fluor? 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bond. The resulting conjugates exhibit brighter fluorescence and greater photostability than the conjugates of many other spectrally similar fluorophores.

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本文由QiyueLh整理，2022年6月1日