IRDye 680RD Infrared Dyes
IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals.
This infrared dye is ideal for labeling proteins, peptides, and antibodies. IRDye 680RD dye conjugates are optimized for protein detection applications, including quantitative Westerns and In-Cell?Western??assays. When used with IRDye 800CW dye-labeled agents, IRDye 680RD dye conjugates enable two-color imaging.
In-Cell Western Assay normalization with IRDye 680RD secondary antibody. A431 cells were treated with 2-fold serial dilutions of EGF (800-1.6 ng/mL). After fixation and permeabilization, cells were probed with rabbit anti-phospho EGFR and Mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070). Column 1: background (no primary antibody); Columns 2-11: 2-fold dilution EGF treatment; Column 12: standard (no EGF treatment).
IRDye 680RD NHS Ester
IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including Western blotting, plate-based assays, protein arrays, tissue section imaging, and molecular activity measurements.
Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.
IRDye 680RD Maleimide
IRDye 680RD Maleimide will label molecules with free sulfhydryl (-SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.
Chemical Formula: C45H48CIN5Na2O12S3
Molecular Weight: 1028.51 g/mol
Exact Mass: 1027.19
IRDye 680RD Carboxylate
Assays that use IRDye 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.
Chemical Formula: C39H41CIN3Na3O11S3
Molecular Weight: 928.37 g/mol
Exact Mass: 927.13
IRDye 680RD Carboxylic acid
IRDye 680RD infrared dye is the near-infrared fluorescent dye of choice for small animal imaging applications in the 700 nm wavelength and provides excellent clearance profiles in animals.
This infrared dye is ideal for labeling proteins, peptides, and antibodies. IRDye 680RD dye conjugates are optimized for protein detection applications, including quantitative Westerns and In-Cell?Western??assays. When used with IRDye 800CW dye-labeled agents, IRDye 680RD dye conjugates enable two-color imaging.
In-Cell Western Assay normalization with IRDye 680RD secondary antibody. A431 cells were treated with 2-fold serial dilutions of EGF (800-1.6 ng/mL). After fixation and permeabilization, cells were probed with rabbit anti-phospho EGFR and Mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070). Column 1: background (no primary antibody); Columns 2-11: 2-fold dilution EGF treatment; Column 12: standard (no EGF treatment).
IRDye 680RD 紅外染料
IRDye 680RD 紅外染料是小動(dòng)物成像應(yīng)用的近紅外熒光染料，波長(zhǎng)為 700 nm，可在動(dòng)物體內(nèi)提供出色的曲線(xiàn)。
這種紅外染料是標(biāo)記蛋白質(zhì)、肽和抗體的選擇。 IRDye 680RD 染料偶聯(lián)物針對(duì)蛋白質(zhì)檢測(cè)應(yīng)用進(jìn)行了優(yōu)化，包括定量 Westerns 和
In-Cell?Western??測(cè)定。當(dāng)與 IRDye 800CW 染料標(biāo)記試劑一起使用時(shí)，IRDye 680RD 染料結(jié)合物可實(shí)現(xiàn)雙色成像。
使用 IRDye 680RD 二抗進(jìn)行 In-Cell Western Assay 化。 A431 細(xì)胞用 2 倍連續(xù)稀釋的 EGF
(800-1.6 ng/mL) 處理。固定和透化后，用兔抗磷酸化 EGFR 和小鼠抗 ERK2 探測(cè)細(xì)胞，然后用 IRDye 800CW 山羊抗兔
IgG(P/N 926-32211)和 IRDye 680RD 山羊抗小鼠 IgG(P/N 926-68070)。第 1 列：背景(無(wú)一抗);第 2-11
列：2 倍稀釋 EGF 處理;第 12 列： 品(無(wú) EGF 處理)。
IRDye 680RD NHS 酯
LI-COR Biosciences 的 IRDye 紅外染料可供研究人員使用近紅外 (NIR) 熒光開(kāi)發(fā)應(yīng)用。這些的染料用于各種 NIR
成像應(yīng)用，包括蛋白質(zhì)印跡、基于板的測(cè)定、蛋白質(zhì)陣列、組織切片成像和分子活性測(cè)量。
NHS 酯化學(xué)用于生產(chǎn)用 LI-COR IRDye 紅外染料標(biāo)記的定制探針。 NHS
酯反應(yīng)基團(tuán)提供了標(biāo)記伯胺和仲胺的功能，例如蛋白質(zhì)中的賴(lài)氨酸殘基。
IRDye 680RD 馬來(lái)酰亞胺
IRDye 680RD 馬來(lái)酰亞胺將標(biāo)記具有游離巰基 (-SH) 基團(tuán)的分子，例如蛋白質(zhì)中的半胱氨酸殘基。綴合可以在生理pH下進(jìn)行。
化學(xué)式：C45H48CIN5Na2O12S3
分子量：1028.51 g/mol
確質(zhì)量：1027.19
IRDye 680RD 羧酸鹽
使用 IRDye 680RD 偶聯(lián)物的檢測(cè)(例如小動(dòng)物成像和細(xì)胞結(jié)合檢測(cè))可能需要“僅染料”對(duì)照來(lái)控制染料的潛在影響或保留。
IRDye 680RD 的羧酸鹽(非反應(yīng)性)形式是的對(duì)照。
化學(xué)式：C39H41CIN3Na3O11S3
分子量：928.37 g/mol
que質(zhì)量：927.13
IRDye 680RD 羧酸
IRDye 680RD 紅外染料是小動(dòng)物成像應(yīng)用的近紅外熒光染料，波長(zhǎng)為 700 nm，可在動(dòng)物體內(nèi)提供出色的曲線(xiàn)。
這種紅外染料是標(biāo)記蛋白質(zhì)、肽和抗體的選擇。 IRDye 680RD 染料偶聯(lián)物針對(duì)蛋白質(zhì)檢測(cè)應(yīng)用進(jìn)行了優(yōu)化，包括定量 Westerns 和
In-Cell?Western??測(cè)定。當(dāng)與 IRDye 800CW 染料標(biāo)記試劑一起使用時(shí)，IRDye 680RD 染料結(jié)合物可實(shí)現(xiàn)雙色成像。
使用 IRDye 680RD 二抗進(jìn)行 In-Cell Western Assay 化。 A431 細(xì)胞用 2 倍連續(xù)稀釋的 EGF
(800-1.6 ng/mL) 處理。固定和透化后，用兔抗磷酸化 EGFR 和小鼠抗 ERK2 探測(cè)細(xì)胞，然后用 IRDye 800CW 山羊抗兔
IgG(P/N 926-32211)和 IRDye 680RD 山羊抗小鼠 IgG(P/N 926-68070)。第 1 列：背景(無(wú)一抗);第 2-11
列：2 倍稀釋 EGF 處理;第 12 列： 品(無(wú) EGF 處理)。
相關(guān)產(chǎn)品：
IRDye 800CW熒光標(biāo)記氨酸NeuGc
IRDye 800CW熒光標(biāo)記唾液酸NeuAc
IRDye 800CW熒光標(biāo)記甘露糖胺Mannosamines
IRDye 800CW熒光標(biāo)記N-乙酰-D-甘露糖胺 一水合物N-Acetyl-D-mannosamine Monohydrate
IRDye 800CW熒光標(biāo)記N-羥乙?；?D-甘露糖胺五乙酸酯Ac5ManNGc
IRDye 800CW熒光標(biāo)記二糖， Disaccharide
IRDye 800CW熒光標(biāo)記苦杏仁苷，Amygdalin
IRDye 800CW熒光標(biāo)記異乳糖，Allolactose
IRDye 800CW熒光標(biāo)記D-纖維二糖，D-(+)-Cellobiose
IRDye 800CW熒光標(biāo)記八醋酸纖維素，α-D-Cellobiose Octaacetate
IRDye 800CW熒光標(biāo)記Crocin (Gardenia Fruits Extract)
IRDye 800CW熒光標(biāo)記地奧司明Diosmin
IRDye 800CW熒光標(biāo)記地奧斯明Barosmin
IRDye 800CW熒光標(biāo)記西紅花苷Crocin
IRDye 800CW熒光標(biāo)記朝藿定C，Epmedin C
IRDye 800CW熒光標(biāo)記寶藿甙VI，Baohuoside VI
IRDye 800CW熒光標(biāo)記龍膽二糖，Gentiobiose
IRDye 800CW熒光標(biāo)記甘草酸，Glycyrrhizin/Glycyrrhizic Acid
IRDye 800CW熒光標(biāo)記肌醇半乳糖苷，Galactinol
本文由QiyueLh整理，2022年6月1日
關(guān)鍵字： IRDye 680RD;
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