IRDye 680LT Infrared Dyes

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes, such as Alexa Fluor? 680. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging systems with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. IRDye 680LT exhibits low background at near-infrared wavelengths resulting in higher signal-to-noise ratios.

IRDye 680LT dye conjugates can be used for quantitative Western blotting and fluorescence microscopy. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.

IRDye 680LT NHS Ester

IRDye infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence.

Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

Chemical Formula: C54H52N3Na3O22S6

Molecular Weight: 1402.32 g/mol

Exact Mass: 1401.09

IRDye 680LT Maleimide

IRDye 680LT Maleimide will label molecules with free sulfhydryl (–SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.

Chemical Formula: C56H55N5Na5O21S6

Molecular Weight: 1427.37 g/mol

Exact Mass: 1426.12

Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In?Cell Western? Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

IRDye 680LT Carboxylic acid

IRDye 680LT dye is highly soluble in water and is significantly brighter and more photostable than many other 700 nm near-infrared dyes, such as Alexa Fluor? 680. The spectral characteristics of IRDye 680LT are well suited for use on LI-COR imaging systems with absorbance and emission maxima in aqueous solution and methanol of 676 and 693 nm, respectively. IRDye 680LT exhibits low background at near-infrared wavelengths resulting in higher signal-to-noise ratios.

IRDye 680LT dye conjugates can be used for quantitative Western blotting and fluorescence microscopy. When using IRDye 680LT secondary antibodies for Western blot detection, it is critical to follow the recommendations in the pack insert. Dilution optimization may be required to achieve best results.

　　IRDye 680LT 紅外染料

　　IRDye 680LT 染料易溶于水，比許多其他 700 nm 近紅外染料(如 Alexa Fluor? 680)更亮且更耐光。IRDye 680LT 的光譜適合用于具有以下的 LI-COR 成像系統(tǒng)水溶液和甲醇中的大吸光度和發(fā)射大值分別為 676 和 693 nm。 IRDye 680LT 在近紅外波長下表現(xiàn)出低背景，從而導(dǎo)致更高的信噪比。

　　IRDye 680LT 染料偶聯(lián)物可用于定量蛋白質(zhì)印跡和熒光顯微鏡檢查。使用 IRDye 680LT 二抗進(jìn)行蛋白質(zhì)印跡檢測時(shí)，遵循包裝插頁中的建議至關(guān)重要?？赡苄枰♂寖?yōu)化以獲得佳結(jié)果。

　　IRDye 680LT NHS 酯

　　LI-COR Biosciences 的 IRDye 紅外染料可供研究人員使用近紅外 (NIR) 熒光開發(fā)應(yīng)用。

　　標(biāo)準(zhǔn) NHS 酯化學(xué)用于生產(chǎn)用 LI-COR IRDye 紅外染料標(biāo)記的定制探針。 NHS 酯反應(yīng)基團(tuán)提供了標(biāo)記伯胺和仲胺的功能，例如蛋白質(zhì)中的賴氨酸殘基。

　　化學(xué)式：C54H52N3Na3O22S6

　　分子量：1402.32 g/mol

　　質(zhì)量：1401.09

　　IRDye 680LT 馬來酰亞胺

　　IRDye 680LT 馬來酰亞胺將標(biāo)記具有游離巰基 (-SH) 基團(tuán)的分子，例如蛋白質(zhì)中的半胱氨酸殘基。綴合可以在生理pH下進(jìn)行。

　　化學(xué)式：C56H55N5Na5O21S6

　　分子量：1427.37 g/mol

　　質(zhì)量：1426.12

　　注意：IRDye 680LT 染料產(chǎn)品不應(yīng)用于小動(dòng)物體內(nèi)成像或 In-Cell Western? 檢測。較高水平的熒光強(qiáng)度會(huì)產(chǎn)生高背景，使其不利于在這些應(yīng)用中使用。我們建議在這些應(yīng)用中使用 IRDye 680RD 染料產(chǎn)品。

　　IRDye 680LT 羧酸

　　IRDye 680LT 染料易溶于水，比許多其他 700 nm 近紅外染料(如 Alexa Fluor? 680)更亮且更耐光。IRDye 680LT 的光譜適合用于具有以下的 LI-COR 成像系統(tǒng)水溶液和甲醇中的大吸光度和發(fā)射大值分別為 676 和 693 nm。 IRDye 680LT 在近紅外波長下表現(xiàn)出低背景，從而導(dǎo)致更高的信噪比。

　　IRDye 680LT 染料偶聯(lián)物可用于定量蛋白質(zhì)印跡和熒光顯微鏡檢查。使用 IRDye 680LT 二抗進(jìn)行蛋白質(zhì)印跡檢測時(shí)，遵循包裝插頁中的建議至關(guān)重要?？赡苄枰♂寖?yōu)化以獲得佳結(jié)果。

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IRDye 800CW熒光標(biāo)記柚皮苷Naringin

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本文由QiyueLh整理，2022年6月1日