α-galactosidase
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α-galactosidase ??
- ?? ??
- 2-8°C
- ??? ??
- ?? ???
- ??
- Colorless to light yellow
- Specific Activity
- ≥9units/mg protein
- CAS ??????
- 9025-35-8
- ?? ? ?? ??
- ?? ? ???? ?? (GHS)
| ????(GHS): |
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| ?? ?: | Warning | ||||||||||||||||||||||||||||
| ??·?? ??: |
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| ??????: |
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α-galactosidase C??? ??, ??, ??
????
(1) ?? : ? ??? ?????? ?? ??? ?, ? ?? 4.0ppm ????? ??.
(2) ? : ? ?? 5.0g? ??? ??????? ?? ?????????????? ?? ??? ?, ? ?? 5.0ppm ????? ??.
(3) ???? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ????? ?? ??? ?, ?? 1g? 30 ????? ??.
(4) ???? : ? ??? 「??? ?? ? ??」 ?????? ?????? ? ????????? ?? ??? ?, ??(-)??? ??.
(5) ??? : ? ??? 「??? ?? ? ??」?????? ?????? ? ???? ?? ??? ?, ??(-)??? ??.
????
? ? ??? ?????? ?? ??? ? ??? ????? ??.
??
? ? ??? Aspergillus niger? ????? ??? ????. ??, ????, ???? ?? ??? ???, ??? ?? ??? ? ??.
? ? ??? ??? ??? ??? α-D-????? ??? ??????.
?????
???? : ? ????? ?? 37℃, pH 5.5?? p-nitrophenyl-α-D-galactopyranoside ??? 15??? ????? ??? ?? ??.
????? ?? : ??? ?? ?? ?? ??? 1mL? 0.001～0.003 galactosidase unit? ????? ????? ????.
???? : 20×150mm ??? ?? ??? ? ?? ??? ?? ???? 2.0mL?? ??? 37±0.2℃? ????? 15? ?? ???? ??? ?????. ????? ??? ???? ?????? 1.0mL?? ??? ??? ??? ??? ????. ??? 15? ? ????????(pH 9.7) 5.0mL? ? ???? ??? ??? ??? ? ?? ????? ?? 405nm?? ???(AS)? ????. ?? ??????? ?????? 1mL?? ??? ??? ????????(pH 9.7) 5.0mL? ??? ??? ??? ?? ???? 2.0mL? ? ???? ??? ??? ?? ??????? ???? ???? ?? 405nm?? ???(AB)? ????. ??? ???? ?? ???? ??? ???.
??(GALU/g) =(AS–AB) × F/ε × T × M
AS : ????? ???
AB : ???? ???
F : ????? ????
T : ?? ??(min)
M : ???? 1mL? ??? ??? ?(g)
ε : ?? 4-???????? ?? ??? ????
??? ?? : 1 α-Galactosidase unit(GALU)? ?????? ??? ?? 1μmol? p-?????? ????? ??? ???.
? ?
??????(pH 5.5)
A? : 11.55mL? ???? ?? ?? 1,000mL? ??.
B? : ????? 16.4g? ?? ?? 1,000mL? ??.
A? 7.5mL? B? 42.5mL? ???? A? ?? B?? ???? pH 5.5? ???? ?? ??? ??? 1,000mL? ??.
???? : p-?????-??-D-????????(p-nitrophenyl-α-D-galactopyranoside) 0.0383g? ??????? ???? ???? 100mL? ??.
???????? : ????? 47.63g? ???? ?? ????? ???? ?? ?? 4N ?????? 20mL? ???. pH??? 4N ???????? 9.7?? ??? ? ???? 2,000mL? ??.
4-????????? : ?? ???? 4-????? 68.83mg? ??? ?? ?? ?? 1,000mL? ??. ? ?? 1mL?? 0.5μmol? ?????? ????.
?? 4-??????? : ? ?? ???? 4-????? ????? ?? 4mL, 8mL ? 16mL? ??? ?? ??? 50mL? ????. ?? ???? 1mL? 4-?????? ?? 0.04, 0.08 ? 0.16μmol? ????. ??? 5?? ??? ?? ???? 2.0mL?? ??? ?? ?? ?? ???? ?? ?? ??? ?? 4-??????? 1mL?? ? ?? ???? ??? ???, ??? ????? ???? ?? ? 1.0mL? ???. ?? ??? ???? ???????? ?? 5.0mL?? ?? ? ??? ??? ?? ?? ??? ?? ?? ????? ?? ?? 1cm, ?? 405nm?? ??? ???? ???? 4-????? μmol?? ?? ???? ????. ? ???? ???? 4-????? ??(μmol/mL)? ??? ?? 4-???????? ?? ????(ε)? ???.
ε = AN/C
AN : 4-????? ????? ???
C : 4-?????? ??(μmol/mL)
???? ?? 18.3 ???? ???? ??.??? ??
Derived from Mortierella vinaceae var. raffinoseutilizer.??
Alkaline α-Galactosidase I was used to assay enzyme activity with 2 mmp-nitrophenyl-α-d-galactoside as substrate at pH 6.5 to compare with the enzyme activity of α-Gal A isolated and purified from Sf-9 insect cells infected with a recombinant baculovirus encoding normal α-Gal A gene.?? ??
Alkaline α-Galactosidase I is a glycoside hydrolase, separated from the extracts of melon fruits. The activity of this enzyme is found to increase during the early stages of ovary development and fruit set.α-galactosidase ?? ?? ? ???
???
?? ??
α-galactosidase ?? ??
| ??? | ?? | ??? | ?? | ?? ? | ?? |
|---|---|---|---|---|---|
| Hebei Chuanghai Biotechnology Co., Ltd | +8615531157085 |
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| Shanghai Zheyan Biotech Co., Ltd. | 18017610038 |
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| career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29867 | 58 |
| Xi'an Kono chem co., Ltd., | 029-86107037 13289246953 |
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| Shandong chuangyingchemical Co., Ltd. | 18853181302 |
sale@chuangyingchem.com | CHINA | 5906 | 58 |