2-Nitrophenyl-β-D-galaktopyranosid
|
|
|
- CAS-Nr.
- 369-07-3
- Bezeichnung:
- 2-Nitrophenyl-β-D-galaktopyranosid
- Englisch Name:
- 2-Nitrophenyl-beta-D-galactopyranoside
- Synonyma:
- ONPG;o-NPG=ONPG;2-Nitrophenyl;ONPG Solution;ONP-BETA-D-GAL;2-Nitrophenyl-beta-D;2-Nitrophenyl-b-D-galactopy;2-Nitrophenyl hexopyranoside;ONP BETA-D-GALACTOPYRANOSIDE;2-Nitrophenyl-β-D-galactoside
- CBNumber:
- CB1366392
- Summenformel:
- C12H15NO8
- Molgewicht:
- 301.25
- MOL-Datei:
- 369-07-3.mol
|
2-Nitrophenyl-β-D-galaktopyranosid Eigenschaften
- Schmelzpunkt:
- 186-193 °C (dec.)
- alpha
- -70 º (c=1, H2O)
- Siedepunkt:
- 442.39°C (rough estimate)
- Dichte
- 1.3709 (rough estimate)
- Brechungsindex
- -68.8 ° (C=1, H2O)
- storage temp.
- 2-8°C
- L?slichkeit
- DMSO (Slightly), Water (Slightly, Heated, Sonicated)
- pka
- 12.69±0.70(Predicted)
- Aggregatzustand
- Powder
- Farbe
- White to very slightly yellow
- Optische Aktivit?t
- [α]20/D 70±1°, c = 1% in H2O
- Wasserl?slichkeit
- Soluble in water.
- BRN
- 92207
- InChIKey
- KUWPCJHYPSUOFW-RMPHRYRLSA-N
- CAS Datenbank
- 369-07-3(CAS DataBase Reference)
- NIST chemische Informationen
- Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3)
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
|
| Alarmwort |
Warnung |
| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H315 |
Verursacht Hautreizungen. |
Hautreizung |
Kategorie 2 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P280, P302+P352, P321,P332+P313, P362 |
| H319 |
Verursacht schwere Augenreizung. |
Schwere Augenreizung |
Kategorie 2 |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
P264, P280, P305+P351+P338,P337+P313P |
| H335 |
Kann die Atemwege reizen. |
Spezifische Zielorgan-Toxizit?t (einmalige Exposition) |
Kategorie 3 (Atemwegsreizung) |
Warnung |
![GHS hazard pictograms]() src="/GHS07.jpg" width="20" height="20" /> |
|
|
| Sicherheit |
| P261 |
Einatmen von Staub vermeiden. |
| P271 |
Nur im Freien oder in gut belüfteten R?umen verwenden. |
| P280 |
Schutzhandschuhe/Schutzkleidung/Augenschutz tragen. |
| P302+P352 |
BEI BERüHRUNG MIT DER HAUT: Mit viel Wasser/... (Hersteller kann, falls zweckm??ig, ein Reinigungsmittel angeben oder, wenn Wasser eindeutig ungeeignet ist, ein alternatives Mittel empfehlen) waschen. |
| P304+P340 |
BEI EINATMEN: Die Person an die frische Luft bringen und für ungehinderte Atmung sorgen. |
| P305+P351+P338 |
BEI KONTAKT MIT DEN AUGEN: Einige Minuten lang behutsam mit Wasser spülen. Eventuell vorhandene Kontaktlinsen nach M?glichkeit entfernen. Weiter spülen. |
| P312 |
Bei Unwohlsein GIFTINFORMATIONSZENTRUM/Arzt/... (geeignete Stelle für medizinische Notfallversorgung vom Hersteller/Lieferanten anzugeben) anrufen. |
| P403+P233 |
An einem gut belüfteten Ort aufbewahren. Beh?lter dicht verschlossen halten. |
|
2-Nitrophenyl-β-D-galaktopyranosid Chemische Eigenschaften,Einsatz,Produktion Methoden
R-S?tze Betriebsanweisung:
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-S?tze Betriebsanweisung:
S22:Staub nicht einatmen.
S24/25:Berührung mit den Augen und der Haut vermeiden.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
2-Nitrophenyl-β-D-galactopyranoside (ONPG) is a colorimetric and spectrophotometric substrate for detecting β-galactosidase activity. This compound is usually colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophenol. The latter compound has a yellow color that can be used to check for enzyme activity utilizing a colorimetric assay (at 420 nm wavelength). β-Galactosidase is required for lactose utilization, so the intensity of the color produced can be used to measure the enzymatic rate. Though ONPG mimics lactose and is hydrolyzed by β-galactosidase, it cannot act as an inducer for the lac operon. Without another lactose analog that can act as an inducer, such as isopropyl β-D-1-thiogalactopyranoside (IPTG), β-galactosidase will not be transcribed and ONPG will not be hydrolyzed.
Chemische Eigenschaften
White Crystalline Solid
Verwenden
2-Nitrophenyl-beta-D-galactopyranoside is a β-Galactosidase substrate for colorimetric and EIA applications; counterpart of widely employed pNPP/alkaline phosphatase substrate.
Definition
ChEBI: A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.
Allgemeine Beschreibung
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
l?uterung methode
Purify 2-nitrophenyl--D-galactopyranoside by recrystallisation from EtOH. [Seidman & Link J Am Chem Soc 72 4324 1950, Snyder & Link J Am Chem Soc 75 1758 1953]. It is a
chromogenic substrate for -galactosidases [Jagota et al. J Food Sci 46 161 1981]. [Beilstein 17/7 V 52.]
2-Nitrophenyl-β-D-galaktopyranosid Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
2-Nitrophenyl-β-D-galaktopyranosid Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 470)Lieferanten
369-07-3(2-Nitrophenyl-β-D-galaktopyranosid)Verwandte Suche:
- 2-Nitrophenyl hexopyranoside
- 2-Nitrophenyl-beta-delta-galactopyranoside
- beta-D-Galactopyranoside, 2-nitrophenyl
- beta-d-galactopyranoside,2-nitrophenyl
- Galactopyranoside, o-nitrophenyl, beta-D-
- o-Nitrophenyl beta-galactoside
- 2-Nitrophenyl-β-D-galactopyranoside
- ONPG o-Nitrophenyl-?-D-galactoside
- 2'-Nitrophenyl-D-galactopyranoside (ONPG)
- 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE, 99+%
- 2-Nitrophenyl-B-D-Galactopyranoside(Onpg)
- 2-Nitrophenyl-Beta-D-Galactopyranside
- O-Nitrophenyl-Beta-D-GalactopyranosideA.R.
- o-NPG=ONPG
- 2-Nitrophenyl
- Nitrophenyl-b-D-galactopyranoside
- 2-nitrophenyl-á-d-galactopyranoside
- ONPG（2-Nitrophenyl-beta-D-galactopyranoside
- 2-Nitrophenyl b-D-galactoside-(1,5), ONPG
- 2-Nitrophenyl-β-D-galactoside
- 2-Nitrophenyl β-D-Galactopyranoside [Substrate for β-D-Galactosidase]
- ortho-Nitrophenyl-β-galactoside
- (2R,3R,4S,5R,6S)-2-(hydroxyMethyl)-6-(2-nitrophenoxy)tetrahydro-2H-pyran-3,4,5-triol
- Ortho nitroalpha -D-glucopyranoside
- The 2- nitrophenyl beta-D- galactopyranoside
- ortho-nitrophenyl β-D-galactopyranoside
- 2-nitrophenyl β-d-[1-13C]galacto-pyranoside
- 2-nitrophenyl β-d-[2-13C]galacto-pyranoside
- TOLUIDINE BLUE O HIGH PURITY GRADE
- 2-Nitrophenyl--D-galactopyranoside for synthesis
- 2-Nitrophenyl-β-D-Galactopyranoside (plant origin)
- 2-NITROPHENYL-B-D-GALACTOPYRANOSIDE
- 2-NITROPHENYL-B-D-GALACTOSIDE
- 2'-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE
- 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE
- 2-NITROPHENYL-BETA-D-GALACTOSIDE
- 2-NITROPHENYL BETA-D-GALACTOSIDE-(1,5)
- 2-NITROPHENYL-BETA-D-GALAKTOPYRANOSIDE
- 2-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE (ONPG)
- 2''-NITROPHENYL-SS-D-GALACTOPYRANOSIDE (ONPG)
- 2-Nitrophenyl-β-D-galactopyranoside, 98+%
- 2-Nitrophenyl beta-D-Galactopyranoside [Substrate for beta-D-Galactosidase]
- o-NITROPHENYL-b-D-GALACTOPYRANOSIDE extrapure
- o-Nitrophenyl β-D-galactopyranoside, ONPG
- (2R,3R,4S,5R,6S)-2-(Hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
- O-Nitrophenyl-beta-d-galactopyranoside 99%
- 2-Nitrophenyl-^b-D-galactopyranoside, 98+%
- 2-Nitrophenyl-b-D-galactopy
- 2-Nitrophenyl-b-D-glucosidase
- 2-Nitrophenyl-beta-D-galactopyranoside, 98+%
- 2-Nitrophenyl β-D-galactopyranoside,o-Nitrophenyl β-D-galactopyranoside, ONPG
- 2-Nitrophenyl-beta-D
- 2-NITROPHENYL-SS-D-GALACTOPYRANOSIDE
- 2-Nitrophenyl b-D-galactopyranoside - non-animal origin
- ONP-BETA-D-GAL
- ONP BETA-D-GALACTOPYRANOSIDE
- O-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE
- O-NITROPHENYL-B-D-GALACTOPYRANOSIDE
