9045-22-1
基本信息
肝素鋰
肝素鋰鹽
肝素鋰 高效價
肝素鋰(注射級)
肝素鋰(豬腸粘液)
肝素 鋰鹽 來源于豬腸粘膜
肝素鋰, 170 單位/毫克
肝素鋰,~200 UNITS/MG
肝素鋰/HEPARIN LITHIUM
LithiuM heparin
HEP, LI, PORCINE
Heparin,lithiumsal
HEPARIN LITHIUM SALT
Erucic Acid 112-86-7
PORCINE HEPARIN, LITHIUM
Heparin, LithiuM salt USP
HEPARIN PORCINE LITHIUM SALT
HEPARIN, LITHIUM SALT, PORCINE
物理化學(xué)性質(zhì)
安全數(shù)據(jù)
常見問題列表
肝素鋰是一種化學(xué)物質(zhì)，外觀是白色至類白色粉末。肝素鋰抗凝血漿與血清中TP、ASO、UA、ALT、Mg、Cl、TC、CRP 檢測結(jié)果比較差異無統(tǒng)計學(xué)意義(P>0.05)。肝素鋰抗凝血漿與血清中HBD、LDH、TBA 檢測結(jié)果比較差異有統(tǒng)計學(xué)意義(P<0.05)。因此除HBD、LDH、TBA 外，肝素鋰抗凝血漿與血清之間的相關(guān)性較好。因此，在生活檢測中采用肝素鋰抗凝血漿代替血清的可行性較高，可將其作為一種重要的檢測方法。
1）粗品肝素酶解：將粗品肝素鈉溶解，調(diào)PH值為7.5?9.0，升溫至50?55℃，加入肝素酶解胰酶，保溫4?5小時，調(diào)PH值 9.0?11，升溫至85℃～90℃后，冷卻，過濾去除沉淀物，濾液用乙醇沉淀，收集沉淀物(A)；
2）去蛋白：步驟1所得沉淀物(A)用水溶解，攪拌，調(diào) PH至9?11，加入蛋白質(zhì)絮凝沉淀劑，攪拌，靜置，過濾去除沉淀，收集濾液(D)，濾液(D)調(diào)PH至11?13值，攪拌，靜置，過濾去除沉淀，收集濾液(E)，濾液(E)調(diào)PH值至中性，用乙醇沉淀，收集沉淀物(B)；
3）氧化脫色：將步驟2所得沉淀物(B)用水溶解，用過氧 化氫氧化24?48小時，調(diào)整PH至10.5?11.5后，過濾去除沉淀物，收集濾液(F)，濾液(F)調(diào)PH值至6.5?7.5，用乙醇沉淀，收集 沉淀物(C)；
4）樹脂交換：將步驟3所得沉淀物(C)用水溶解，加入 LiCl，攪拌溶解，通過樹脂柱進行離子交換，收集過柱流出液，得肝素鋰溶液；
5）沉淀、脫水、干燥：將步驟4所得肝素鋰溶液，過濾，用乙醇沉淀，收集沉淀物(G)，沉淀物(G)脫水，然后真空干 燥，粉碎，得肝素鋰精品。
Antithrombin III
Heparin is a potent anticoagulant drug based on its ability to accelerate the rate at which antithrombin inhibits serine proteases in the blood coagulation cascade. Heparin interactes most tightly with peptides containing a complementary binding site of high positive charge density. Heparin resembles DNA as both are highly charged linear polymers that behave as polyelectrolytes. Heparin is believed to function as an anticoagulant primarily through its interaction with AT III by enhancing AT-III-mediated inhibition of blood coagulation factors, including thrombin and factor Xa. Heparin binds to AT III and thrombin in a ternary complex, increasing the bimolecular rate constant for the inhibition of thrombin by a factor of 2000. Heparin is principally located in the granules of tissue mast cells that are closely associated with the immune response. Heparin makes numerous contacts with both FGF-2 and FGFR-1 stabilizing FGF–FGFR binding. Heparin also makes contacts with the FGFR-1 of the adjacent FGF–FGFR complex, thus seeming to promote FGFR dimerization.