138516-31-1
138516-31-1 結(jié)構(gòu)式
基本信息
RO 31-7549 ACETATE
BISINDOLYLMALEIMIDE VIII ACETATE SALT
BisindolylMaleiMide VIII . AcOH [Ro 31-7549]
3-[1-(3-Aminopropyl)-1H-indol-3-yl]-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione monoacetate
常見(jiàn)問(wèn)題列表
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Rat Brain PKC 158 nM (IC 50 ) |
PKC-α 53 nM (IC 50 ) |
PKC-βI 195 nM (IC 50 ) |
PKC-βII 163 nM (IC 50 ) |
PKC-γ 213 nM (IC 50 ) |
PKC-ε 175 nM (IC 50 ) |
Bisindolylmaleimide VIII acetate (Ro 31-7549 acetate; 5 μM; 8, 12 hours) dramatically increases TRA-8-induced cell death in time-dependent and TRA-8 dose-dependent manners.
Bisindolylmaleimide VIII acetate (5 μM; 6 hours) significantly decreases procaspase-8 at 4 h and completely disappeares at 6 h after the combined treatment with TRA-8.
Apoptosis Analysis
| Cell Line: | 1321N1 cells |
| Concentration: | 5 μM |
| Incubation Time: | 8, 12 hours |
| Result: | Dramatically increased TRA-8-induced cell death in time-dependent and TRA-8 dose-dependent manners. |
Western Blot Analysis
| Cell Line: | 1321N1 cells |
| Concentration: | 5 μM |
| Incubation Time: | 6 hours |
| Result: | Significantly decreased procaspase-8 at 4 h and completely disappeared at 6 h. |
Bisindolylmaleimide VIII acetate (Ro 31-7549 acetate; 100 μg; IP; every other day for three doses) results in nearly complete tumor regression combined toTRA-8. The treatment with Bisindolylmaleimide VIII acetate alone does not induce significant tumor regression.
| Animal Model: |
6-8-week-old female NOD/SCID mice.
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| Dosage: | 100 μg |
| Administration: | IP; every other day for three doses |
| Result: | Resulted in nearly complete tumor regression combined toTRA-8. |