| Identification | Back Directory | [Name]
Pectinase | [CAS]
9032-75-1 | [Synonyms]
Pectase
ultrazym
PeCLinase
PECTINASE
EC 3.2.1.15
IUB: 4.2.2.10
Pectinex 3X L
Pectinase Y-23
MACEROZYME R-10
GLYCANOHYDROLASE
Macerozyme R-200
Pectinex 3XL(R)
Neutral cellulase
Endogalacturonase
Pectinglycosidase
POLYGALACTURONASE
MACERASE PECTINASE
POLYGALACTOURONASE
Pectinex? Ultra SPL
Pectinex Ultra Clear
pectinase EC 3.2.1.15
pectinase preparatIon
MACERASE(R) PECTINASE
MACERASE(TM) PECTINASE
ENDO-POLY-GALACTURONASE
Pectinex(R) Ultra SPL
ASPERGILLUSNIGERPECTINASE
PECTINASE FROM RHIZOPUS SP
POLYGALACTURONASE PECTINASE
PECTINASE, ASPERGILLUS NIGER
PECTINASE FROM MOULD, ~500 U/G
Pectinase, 10 units/Mg protein
PECTINASE FROM ASPERGILLUS NIGER
Macerozyme R-10 from Rhizopus sp.
PECTINASE FROM RHIZOPUS SP. ~5 U/G
PectinaseExAspergillusUsamilMutant
Macerozyme R-10, Polygalacturonase
PECTINASE FROM RHIZOPUS SP. ~20 U/MG
MACERASE(R) PECTINASE, RHIZOPUS SPECIES
PECTINASE FROM RHIZOPUS SP. CRUDE POWDER
pectinase solution from aspergillus niger
Pectinase from Aspergillus niger, ~1 U/m g
poly-(1,4-α-d-galacturonide) glycanohydrolase
POLY-[1-4ALPHA-D-GALACTURONIDE]GLYCONO-HYDROLASE
POLY-(1,4-ALPHA-D-GALACTOURONIDE)GLYCANOHYDROLASE
POLY-[1,4-ALPHA-D-GALACTURONIDE] GLYCANOHYDROLASE
Polygalacturonase solution from Aspergillus niger
PECTINASE FROM ASPERGILLUS NIGER-*PLANT CELL CULT
PECTINASE FROM RHIZOPUS SP. PLANT CELL*C ULTURE TEST
POLYGALACTURONASE, STERILE, FOR PLANT*TI SSUE CULTUR
PECTINASE FROM ASPERGILLUS NIGER SOLUTION IN GLYCEROL
POLY-[1,4-ALPHA-D-GALACTURONIDE]GLYCANOHYDROLASE PECTINASE
PECTINASE (FROM ASPERGILLUS NIGER) 1.0 U MG FOR BIOCHEMISTRY
Endogalacturonase, Poly-(1,4-α-D-galacturonide)-glycanohydrolase
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
Pectinase from Aspergillus niger,Macerozyme R-10, Polygalacturonase
Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
Macerozyme R-200, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
Pectinase from Rhizopus sp.,Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger
Pectinase from Rhizopus sp.,Macerozyme R-10, Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
Pectinase from Aspergillus niger,Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase solution from Aspergillus niger | [EINECS(EC#)]
232-885-6 | [Molecular Formula]
C18H37N(CH3)2 | [MDL Number]
MFCD00131809 |
| Questions And Answer | Back Directory | [Description]
Pectinase is a complex enzyme consisting of pectolyase, pectozyme, and polygalacturonase. It is able to break down pectin, a kind of polysaccharide existing in plant cell wall. Pectin is It functions optimally at temperatures between 45 and 55 °C and pH between 3 and 6.5. Owing to this property, Pectinase is often used in processes demanding the degradation of plant materials such as speeding up the extraction process of fruit juice. More importantly, through breaking down pectin, which is an important diet component of human being, pectinase can positively affect the digestive system, maintain intestinal bacteria balance and promote colon health. Pectinase is also useful during the production of wine for aiding the extraction of flavours from the mash. Pectinase is also used for retting. It is usually obtained from the fermentation of fungi such as Aspergillus Niger.
 | [Sources]
Many molds and small amounts of bacteria and yeasts can produce pectinase, mainly Aspergillus and Bacillus, others including Penicillium (Penicillium expansum, Penicillium griseoroseum, etc), Sclerotium rolfsii, Rhizoctonia solani, Mucor pusilus, Mucor mucedo, Cloctridium thermosaccharolyticum, Rhizopus stolonifer, Aureobasiduim pullulans, Neurospora rassa, Sporotrichum thermophile, etc. As Aspergillus niger in fungi belongs to GRAS (General Regarded As Safe), its metabolites are safe, so currently marketed food grade pectinase is mainly from Aspergillus niger, and the optimum pH value is generally in the acidic range. With the continuous improvement of molecular biology techniques, gene cloning technology can also be used to achieve expression of pectinase in other microbial hosts. | [Physical properties]
The molecular weight of pectinase is generally between 20kD and 60kD, and most of them exist as monomeric enzymes. Pectinase activity is usually in the range of pH 3.0 to 9.0, with an isoelectric point of 4.0 to 9.0. Among them, an optimum pH of a hydrolase is 4.0 to 6.5, and its action does not require the participation of Ca2+. The optimum pH of the penctin lyase is 8.0 to 10.0, and its action requires Ca2+ involvement. The pectinase produced by different strains has different properties. Common purification methods of pectinase include ammonium sulfate precipitation, acetone precipitation, ion exchange chromatography and gel filtration chromatography.
| [Classification]
Pectinase can be divided into four types: protopectinase, polygalacturonase, penctin lyase (PL), and pectinesterase (PE). Protopectalase can promote the dissolution of pectin. According to its catalytic mechanism, protopectinase is divided into two types: Type A pectinase and Type B pectinase. The former mainly acts on the inner polygalacturonic acid region of protopectin. The latter mainly acts on the outer polysaccharide chains of the galacturonic acid chains and cell wall components. Polygalacturonase is a kind of pectinase which promotes the hydrolysis of galacturonic acid chain under water environment. | [Uses]
Pectinase, Aspergillus niger is used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation. It is used in the enzyme preparation. Used mostly to reduce viscosity, to improve filtration to clarify products, to avoid particle sedimentation and to prevent pectin gel formation in the manufacture of fruit juice and wine. | [Application]
An enzyme found in plant cells that breaks down the polysaccharide pectin. In life, this enzyme is involved in various metabolic functions of the plant, including the ripening process. In the kitchen, cooks use this enzyme to break down pectin to soften plant foods as a way to modify texture, make juicing easier, or to assist in the clarification of plant juices. Pectinase is also commonly used in wine making to get rid of cloudiness. | [Preparation]
Pectinase occurs throughout the natural world, but microbes are the primary tool through which it is made for commercial purposes. Producing microbial pectinase occurs mostly by submerging bacteria in a liquid that is continuously agitated, or via solid-state fermentation, which involves growing the microbes on a substrate. The microbes secrete the lipase, which is then purified, homogenized, and crystallized for sale. | [References]
http://www.xtend-life.com/information/ingredients/pectinase
https://en.wikipedia.org/wiki/Pectinase
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| Hazard Information | Back Directory | [General Description]
We are committed to bringing you?Greener?Alternative Products, which adhere to one or more of The 12 Principles of?Greener?Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research. | [Biochem/physiol Actions]
Pectolytic enzyme preparation produced from a selected strain of Aspergillus niger: contains mainly pectintranseliminase, polygalacturonase, and pectinesterase and small amounts of hemicellulases and cellulases. Pectinases hydrolyses pectin, which is a component of the cell wall. They may attack methyl-esterified pectin or de-esterified pectin. It is a source of pectinase activity, also containing cellulase and hemicellulase activities.Pectinase catalyzes the random hydrolysis of a-(1-4)-Dgalactosiduronic linkages in pectin and other galacturonans. |
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