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ChemicalBook--->CAS DataBase List--->7365-45-9

7365-45-9

7365-45-9 Structure

7365-45-9 Structure
IdentificationMore
[Name]

HEPES
[CAS]

7365-45-9
[Synonyms]

2-[4-(2-HYDROXYETHYL)-1-PIPERAZINE]ETHANESULFONIC ACID
2-[4-(2-HYDROXYETHYL)-1-PIPERAZINYL]ETHANESULFONIC ACID
4-(2-HYDROXYETHYL)-1-PIPERAZINEETHANESULFONIC ACID
4-(2-HYDROXYETHYL)PIPERAZINE-1-ETHANESULFONIC ACID
GOOD'S BUFFER
HEPES
HEPES-ACID
HEPES BUFFER
HEPES BUFFERED SALINE
HEPES BUFFER PH 7.5
HEPES BUFFER PH 8.0
LABOTEST-BB LT00138109
LABOTEST-BB LT00233156
N-(2-HYDROXYETHYL)PIPERAZINE N-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANESULFONIC ACID
N-2-HYDROXYETHYLPIPERAZINE-N'-2-ETHANE SULPHONIC ACID
4-(2-hydroxyethyl)-1-piperazineethanesulfonicaci
hepesfreeacid
N-(2-Hydroxyethyl)piper
4-(2-Hydroxyethyl)-1-piperazineethanesulphonic acid
[EINECS(EC#)]

230-907-9
[Molecular Formula]

C8H18N2O4S
[MDL Number]

MFCD00006158
[Molecular Weight]

238.3
[MOL File]

7365-45-9.mol
Chemical PropertiesBack Directory
[Appearance]

White crystalline powder
[Melting point ]

234-238 °C
[Boiling point ]

408℃[at 101 325 Pa]
[density ]

1.07 g/mL at 20 °C
[vapor pressure ]

0Pa at 25℃
[refractive index ]

n20/D 1.339
[storage temp. ]

2-8°C
[solubility ]

H2O: 1 M at 20 °C, clear, colorless
[form ]

powder
[color ]

White
[PH]

5.0-6.5 (1Maqueous solution)
[PH Range]

7.0 - 7.6
[pka]

7.5(at 25℃)
[Stability:]

Stable. Combustible. Incompatible with strong oxidizing agents. Protect from moisture.
[Water Solubility ]

Soluble
[λmax]

λ: 260 nm Amax: 0.06
λ: 280 nm Amax: 0.04
[Detection Methods]

T,NMR
[Merck ]

14,4654
[BRN ]

883043
[InChIKey]

JKMHFZQWWAIEOD-UHFFFAOYSA-N
[LogP]

-3.85 at 20℃
[CAS DataBase Reference]

7365-45-9(CAS DataBase Reference)
[EPA Substance Registry System]

7365-45-9(EPA Substance)
Safety DataBack Directory
[Hazard Codes ]

Xi
[Risk Statements ]

R36/37/38:Irritating to eyes, respiratory system and skin .
[Safety Statements ]

S24/25:Avoid contact with skin and eyes .
S22:Do not breathe dust .
S36:Wear suitable protective clothing .
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
[WGK Germany ]

1
[RTECS ]

TL6809000
[TSCA ]

Yes
[HS Code ]

29335995
Material Safety Data Sheet(MSDS)Back Directory
[msds information]

2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid(7365-45-9).msds
Hazard InformationBack Directory
[Description]

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
[Chemical Properties]

White crystalline powder
[Uses]

Biological buffer.
[Uses]

Non-toxic to cell. It is used as a hydrogen ion buffer, which can control the constant pH range for long term. The concentration is 10-50mmol/L. Generally, 20mmol/LHEPES in nutrient solution can get buffer ability.
[Definition]

ChEBI: A HEPES that is ethanesulfonate substituted by a 4-(2-hydroxyethyl)piperazin-4-ium-1-yl group at position 2.
[General Description]

HEPES buffered saline is a tissue culture buffer, which is used to maintain the pH of cell culture. It is water soluble and atmosphere independent. HEPES destroys magnesium in sodium chloride solutions.
[Biological Activity]

Multi purpose buffer used in biological research.
[storage]

Room temperature
[Purification Methods]

Crystallise the acid from hot EtOH and water. It is a useful buffer. [Beilstein 23 V 376.]
Spectrum DetailBack Directory
[Spectrum Detail]

HEPES(7365-45-9)MS
HEPES(7365-45-9)IR1
HEPES(7365-45-9)IR2
HEPES(7365-45-9)Raman
Well-known Reagent Company Product InformationBack Directory
[Acros Organics]

HEPESfreeacid,DNase,RnaseandProteasefree,formolecularbiology,99+%(7365-45-9)
[Alfa Aesar]

HEPES, 99%(7365-45-9)
[Sigma Aldrich]

7365-45-9(sigmaaldrich)
[TCI AMERICA]

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic Acid,>99.0%(T)(7365-45-9)
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