| Identification | More | [Name]
6-Aminofluorescein | [CAS]
51649-83-3 | [Synonyms]
5-AMINOFLUORESCEIN
5-AMINOFLUORESCEIN (ISOMER II)
6-AMINO-3',6'-DIHYDROXYSPIRO[ISOBENZOFURAN-1(3H),9'-(9H)XANTHEN]-3-ONE
6-amino-3',6'-dihydroxyspiro[isobenzofuran-1(3h),9'-[9h]xanthene]-3-one
6-AMINOFLUORESCEIN
AMINOFLUORESCEIN ISOMER II
FLUORESCEINAMINE, ISOMER 2
FLUORESCEINAMINE, ISOMER II
6-AMINOFLUORESCEIN, FOR FLUORESCENCE
FLUORESCEINMINE, ISOMER 2,
5-AMINOFLUORESCEIN (ISOMER 2)
Fluoresceine amine isomer II | [EINECS(EC#)]
257-334-7 | [Molecular Formula]
C20H13NO5 | [MDL Number]
MFCD00005051 | [Molecular Weight]
347.32 | [MOL File]
51649-83-3.mol |
| Chemical Properties | Back Directory | [Appearance]
Yellow Solid | [Melting point ]
285 °C (dec.)(lit.) | [Boiling point ]
481.89°C (rough estimate) | [density ]
1.3724 (rough estimate) | [refractive index ]
1.5180 (estimate) | [storage temp. ]
2-8°C
| [solubility ]
methanol: soluble1mg/mL, clear, yellow to very dark yellow-orange | [form ]
Solid | [pka]
9.34±0.20(Predicted) | [color ]
Yellow to Orange | [Usage]
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens | [λmax]
495 nm | [BRN ]
51708 | [Stability:]
Light and Moisture Sensitive | [InChI]
InChI=1S/C20H13NO5/c21-10-1-4-13-16(7-10)20(26-19(13)24)14-5-2-11(22)8-17(14)25-18-9-12(23)3-6-15(18)20/h1-9,22-23H,21H2 | [InChIKey]
YOAWSYSKQHLFPM-UHFFFAOYSA-N | [SMILES]
C12(C3=C(C=C(O)C=C3)OC3=C1C=CC(O)=C3)C1=C(C=CC(N)=C1)C(=O)O2 | [CAS DataBase Reference]
51649-83-3(CAS DataBase Reference) |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
R36/37/38:Irritating to eyes, respiratory system and skin . | [Safety Statements ]
S26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice .
S36:Wear suitable protective clothing . | [WGK Germany ]
3
| [F ]
10-23 | [HS Code ]
29329990 |
| Hazard Information | Back Directory | [Description]
6-Aminofluorescein (6-AF) is a new fluorescence dye with bright colors and high fluorescence intensity. Its chemical structure history contains a nitrogen group and a fluorescent group. The presence of the fluorescent component enables 6-Aminofluorescein to emit a distinct fluorescent signal when excited, making it valuable for fluorescent labeling and imaging. It is widely used in biological science research, chemical analysis and fluorescence microscopy. | [Chemical Properties]
Yellow Solid | [Uses]
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens | [Uses]
Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens. | [Uses]
Glycosaminoglycuronans react with 6-aminofluorescein to yield fluorescent derivatives. | [Biological Functions]
6-Aminofluorescein has been demonstrated to be useful fluorescent labeling reagent for fullerene-based liposome nanostructures termed "buckysomes". 6-aminofluorescein dissolved in 0.1 N NaOH can be quantified from a maximum absorbance at 490 nm. Decarboxylated 6-aminofluorescein dissolved 6 N HCl (prepare by constant boiling at 110° C for 24 h) can be quantified from a maximum absorbance at 454 nm. Aldehyde groups are formed by oxidateive damage to proteins, which can be detected by conjugation to decarboxylated 6-aminofluorescein. 6-Aminofluorescein has been used to determine its ability to potentiate the antiviral activity of poly r (A-U). | [References]
[1] BHARATE G Y, QIN H, FANG J. Poly(Styrene-Co-Maleic Acid)-Conjugated 6-Aminofluorescein and Rhodamine Micelle as Macromolecular Fluorescent Probes for Micro-Tumors Detection and Imaging[J]. Journal of Personalized Medicine, 2022, 12. DOI:10.3390/jpm12101650.
[2] Müller, Werner EG, and Heinz Christoph Schr?der, eds. Biological Response Modifiers—Interferons, Double-Stranded RNA and 2′, 5′-Oligoadenylates. Vol. 14. Springer Science & Business Media, 2012. |
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