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Food Chemistry

Food Chemistry

IF: 8.5
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Ultra-small magnetic Candida antarctica lipase B nanoreactors for enzyme synthesis of bixin-maltitol ester

Published:12 April 2023 DOI: 10.1016/j.foodchem.2023.136132 PMID: 37094396
Danyu Lv , Muyun Wang , Wanjun He , Jieli Wu , Xiaoyue Liu , Yongguang Guan

Abstract

Bixin has desirable bioactivities but poor water solubility, which limits its practical applications. Enzymatic transesterification of methyl to alditol groups in bixin by Candida antarctica lipase B (CALB) improves bixin water solubility. Herein, magnetic CALB nanoreactors with diameter of 11.7?nm and CALB layer thickness of 3.5?nm were developed by covalently linking CALB onto silicon covered Fe3O4 nanoparticles. The CALB loading capacity in nanoreactors achieved 30%. The Michaelis constant (Km) and maximum reaction rate of magnetic CALB nanoreactors were 56.1?mmol/L and 0.2?mmol/(L·min). Magnetic CALB nanoreactors could circularly catalyze bixin-maltitol ester synthesis and keep catalytic efficiency of 62.6% after eight repetitive enzymatic reactions. Additionally, the optimal bixin-maltitol ester synthesis procedure was heating bixin-maltitol mixture at molar ratio of 1:7 in anhydrous 2-methyl-2-butanol-dimethylsulfoxide (8:2, v/v) at 50?°C for 24?h. Bixin-maltitol ester showed improved water solubility at pH 5.5 and 7.0.

Substances (5)

Related products
Procduct Name CAS Molecular Formula Supplier Price
Dimethyl sulfoxide 67-68-5 C2H6OS 1348 suppliers $15.00-$120900.00
2-Methyl-2-butanol 75-85-4 C5H12O 367 suppliers $9.00-$1310.00
Bixin 6983-79-5 C25H30O4 79 suppliers $138.00-$724.93
Candida antarctica lipase B 3 suppliers Inquiry
PLL coating Fe3O4 nanoparticles(10nm) 2 suppliers Inquiry

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