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Plant Physiology

Plant Physiology

IF: 6.5
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The key role of phloroglucinol O-methyltransferase in the biosynthesis of Rosa chinensis volatile 1,3,5-trimethoxybenzene.

Published:1 May 2004 DOI: 10.1104/pp.103.037051 PMID: 15122041
Shuiqin Wu, Naoharu Watanabe, Satoru Mita, Hideo Dohra, Yoshihiro Ueda, Masaaki Shibuya, Yutaka Ebizuka

Abstract

1,3,5-Trimethoxybenzene is a key component of the Chinese rose odor. This compound is synthesized in three successive methylation steps from phloroglucinol, the initial precursor. A novel, to our knowledge, phloroglucinol O-methyltransferase (POMT) characterized here methylates the first step to produce the intermediate 3,5-dihydroxyanisole, while two previously described orcinol O-methyltransferases catalyze the subsequent steps. We isolated POMT from rose petals and determined partial amino acid sequences of the purified enzyme. The full-length POMT cDNA was isolated and expressed in Escherichia coli. Both the native and recombinant POMT exhibited substrate specificity for phloroglucinol. POMT was expressed specifically in floral organs, in accordance with its role as a key enzyme in the synthesis of rose floral scent compounds.

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Related products
Procduct Name CAS Molecular Formula Supplier Price
1,3,5-Trimethoxybenzene 621-23-8 C9H12O3 592 suppliers $7.00-$4173.48
Phloroglucinol 108-73-6 C6H6O3 577 suppliers $13.00-$5058.90
3,5-DIHYDROXYANISOLE HYDRATE 2174-64-3 C7H8O3 154 suppliers $35.20-$1460.20

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