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Journal of pharmaceutical and biomedical analysis

Journal of pharmaceutical and biomedical analysis

IF: 3.1
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Developments in on-line, post separation sample manipulation in the last 22 years: Pharmaceutical and biomedical applications

Published:25 October 2023 DOI: 10.1016/j.jpba.2023.115654 PMID: 37611457
Apostolia Tsiasioti, Paraskevas D. Tzanavaras

Abstract

On-line post separation sample manipulation is a powerful approach increasing the sensitivity and selectivity in chemical analysis. Post separation sample manipulation includes the treatment of the analytes after their separation through a suitable separation technique, mainly liquid chromatography and capillary electrophoresis. Typically, post separation approaches include either the addition of a reagent/solvent to derivatize the analyte/enhance the sensitivity, pH change, or the conversion of the analyte through a photochemical/electrochemical system (reagent–free systems). This review focuses on the developed methods using post-column manipulation of sample with pharmaceuticals and biomedical applications, covering the period from 2000 to midle-2023. Chemistries combined with fluorescence, UV–vis and mass spectrometric detection are discussed employing both liquid chromatography and electrophoretic techniques for separation. Noteworthy instrumental modifications are also discussed.

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