一、試劑盒簡介 本試劑盒是簡單快速從細(xì)胞到cDNA的一步法試劑盒,無需復(fù)雜的RNA提取步驟,試劑盒中含有g(shù)DNA Remover可有效去除基因組DNA,極大降低了基因組DNA的污染。具體過程是:根據(jù)細(xì)胞數(shù),按照80 µl /10萬細(xì)胞的量加Cell Lysis Buffer,吹吸10次,充分裂解細(xì)胞;立即吸出4 - 8 µl細(xì)胞裂解產(chǎn)物,加入0.8 µl gDNA Remover,室溫反應(yīng)5 min,即可去除基因組DNA。然后加入逆轉(zhuǎn)錄試劑,充分混勻后,只需42℃、15 min即可得到cDNA。 二、產(chǎn)品組分
三、保存條件 此試劑盒建議置于-20℃冰箱中保存。 四、試劑盒特點 1、簡單、快速:僅需2步、20 min即可得到cDNA。 2、基因組殘留少:配有g(shù)DNA Remover,可有效去除DNA污染。 3、所需樣品少:最低可提取1000個細(xì)胞。 4、安全無毒:不使用苯酚、氯仿、異丙醇、DEPC水等對身體有害的物質(zhì)。 五、引用該產(chǎn)品的文獻(xiàn) 1、 Sevoflurane affects neurogenesis through cell cycle arrest via inhibiting wnt/β-catenin signaling pathway in mouse neural stem cells Life Sci. 2018 Sep 15;209:34-42. doi: 10.1016/j.lfs.2018.07.054. Epub 2018 Jul 30. https://www.ncbi.nlm.nih.gov/pubmed/?term=Sevoflurane+affects+neurogenesis+through+cell+cycle+arrest+via+inhibiting+wnt%2Fβ-catenin+signaling+pathway+in+mouse+neural+stem+cells 2、 Functional silencing of HSD17B2 in prostate cancer promotes disease progression Clin Cancer Res. 2019 Feb 15;25(4):1291-1301. doi: 10.1158/1078-0432.CCR-18-2392. Epub 2018 Sep 18. https://www.ncbi.nlm.nih.gov/pubmed/?term=Functional+silencing+of+HSD17B2+in+prostate+cancer+promotes+disease+progression 3、 Antiaging Effects of Urolithin A on Replicative Senescent Human Skin Fibroblasts Rejuvenation Res. 2019 Jun;22(3):191-200. doi: 10.1089/rej.2018.2066. Epub 2018 Oct 25. https://www.ncbi.nlm.nih.gov/pubmed/?term=Antiaging+Effects+of+Urolithin+A+on+Replicative+Senescent+Human+Skin+Fibroblasts 4、 The chromatin remodeling subunit Baf200 promotes normal hematopoiesis and inhibits leukemogenesis J Hematol Oncol. 2018 Feb 26;11(1):27. doi: 10.1186/s13045-018-0567-7. https://www.ncbi.nlm.nih.gov/pubmed/?term=The+chromatin+remodeling+subunit+Baf200+promotes+normal+hematopoiesis+and+inhibits+leukemogenesis 5、 Forsythoside A modulates zymosan-induced peritonitis in mice Molecules. 2018 Mar 6;23(3). pii: E593. doi: 10.3390/molecules23030593. https://www.ncbi.nlm.nih.gov/pubmed/?term=Forsythoside+A+modulates+zymosan-induced+peritonitis+in+mice 六、圖示
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