名稱 Western二抗稀釋液
規(guī)格 100ml
存儲 4℃保存，一年有效。
產(chǎn)品簡介
Western二抗稀釋液(Secondary Antibody Dilution Buffer)可以用于Western時二抗(secondary antibody)的稀釋。經(jīng)本W(wǎng)estern二抗稀釋液稀釋的二抗可以在1-2周內(nèi)重復(fù)使用3-5次。
按照每個二抗稀釋10毫升計算，一個包裝的Western二抗稀釋液可以稀釋10個或10次二抗。
使用說明
參考所用的二抗的使用說明，以及一抗的質(zhì)量和樣品中目的蛋白的含量，按照適當(dāng)比例例如1:1000、1:500等比例稀釋二抗。二抗稀釋后即可直接用于Western。一次Western結(jié)束后，可以回收稀釋的二抗，4℃保存，以用于下次的Western，通常在1-2周內(nèi)可以重復(fù)使用3-5次。
注意事項
本W(wǎng)estern二抗稀釋液經(jīng)過濾除菌處理，使用過程中應(yīng)盡量避免細(xì)菌污染。一旦啟用后建議在一個月內(nèi)用完，如果發(fā)現(xiàn)有沉淀或細(xì)菌污染請棄用。
Note:
1) Antibodies diluted using this buffer can be stored at 4 ºC for 6 months without reducing binding activity;
2) Do not use BSA or other serum containing reagents to dilute secondary antibodies since they may bind to BSA or serum therefore reducing antibody affinity;
3) Using TBS to dilute secondary antibodies often produces weaker staining. So use TBS only for the antibodies with high background staining or for alkaline phosphatase conjugated antibodies since phosphate is a inhibitor of alkaline phosphatase;
4) This buffer can also be used for diluting HRP conjugated antibodies since there is no sodium azide in buffer.