Species

Gene ID

Accession

Source

Length

MW

Tag

Rat

25707

P20294

E. coli

199aa

22.7kDa

－

About this Antibody

Name

Recombinant Rat CNTF (Recombinant Rat Ciliary Neurotrophic Factor; rRtCNTF)；重組大鼠人睫狀神經(jīng)營養(yǎng)因子

Synonyms

ciliary neurotrophic factor; HCNTF

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30ng/ml, corresponding to a specific activity of >3.3×10⁴IU/mg.

Physical Appearance

Sterile Filtered lyophilized (freeze-dried) liquid-like powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 2% trehalose.

Endotoxin

Less than 1EU/μg of rRtCNTF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant rat CNTF containing 199 amino acids and it shares 83% and 95%a.a. sequence identity with human and murine CNTF.

Amino Acid Sequence

AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

產(chǎn)品編號

產(chǎn)品名稱

包裝

P6407-5μg

Recombinant Rat CNTF

5μg

P6407-25μg

Recombinant Rat CNTF

25μg

P6407-100μg

Recombinant Rat CNTF

100μg

P6407-1mg

Recombinant Rat CNTF

1mg

-

說明書

1份