Name

Recombinant Human CNTF (Recombinant Human Ciliary Neurotrophic Factor; rHuCNTF)；重組人睫狀神經(jīng)營養(yǎng)因子

Synonyms

ciliary neurotrophic factor; HCNTF

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150ng/ml, corresponding to a specific activity of >6.7×10³IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin

Less than 1EU/μg of rHuCNTF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. It was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. The protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. Recombinant human CNTF containing 200 amino acids and it shares 82% and 83%a.a. sequence identity with mouse and rat CNTF.

Amino Acid Sequence

MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM

產(chǎn)品編號

產(chǎn)品名稱

包裝

KL-5687-5μg

Recombinant Human CNTF

5μg

KL-5687-20μg

Recombinant Human CNTF

20μg

KL-5687-100μg

Recombinant Human CNTF

100μg

KL-5687-1mg

Recombinant Human CNTF

1mg

-

說明書

1份