背景資料

Formation and expansion of the pre-autophagosomal structure in yeast requires the attachment of the ubiquitin-like protein Atg8 via its C-terminal glycine to the amino group of phosphatidylethanolamine (PE), enabling its anchoring to the isolation membrane of the autophagosome. In mammals, Atg8 is represented by at least six orthologs that fall into two subgroups, LC3- and GABARAP-like proteins and the free and PE linked versions of these proteins are often referred to as LC3-I and LC3-II respectively.

Whilst the specific roles for each individual protein have yet to be fully elucidated they have been shown to be involved in binding and selection of ubiqitinated autophagy substrate proteins, via ubiquitin / LC3 binding receptor proteins such as p62, in addition to their role in autophagosome biogenesis. Release of LC3 / GABARAP proteins via their cleavage from the mature autophagosome structure by Atg4 proteases facilitates their recycling and may be a requirement for autophagosome-lysosome fusion to take place.

應(yīng)用類型

該LC3B 多克隆抗體經(jīng)過WB等實驗驗證。推薦使用稀釋比率分別為：WB 1:500-1000。

免疫原

Peptide derived from N-terminal region of human LC3B protein

來源宿主

兔

反應(yīng)性

人，小鼠，大鼠

保存建議

建議在收到LC3B 多克隆抗體產(chǎn)品后可將其置于4度穩(wěn)定保存1個月。-20度穩(wěn)定保存1年，避免反復(fù)凍融，建議分裝保存！