| 名稱(chēng) | 3,4-Dihydroxybenzylamine hydrobromide |
| 描述 | 3,4-Dihydroxybenzylamine hydrobromide (NSC-263475 hydrobromide) inhibits DNA polymerase activity in melanoma cells and displays growth inhibitory activity in melanoma cell lines with varying degrees of tyrosinase activity. |
| 細(xì)胞實(shí)驗(yàn) | Cell Line: Human and Murine Melanoma Cell Lines; Non-Melanoma Cell Lines
Concentration: 1 μM-10 mM
Incubation Time: 48 hours [1] |
| 動(dòng)物實(shí)驗(yàn) | Animal Model: C57BL/6 mice with B6D2F1 cells
Dosage: 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg
Administration: Intraperitoneal injection [2] |
| 體外活性 | 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) inhibits the growth of all melanoma cell lines (IC50 = 100 μM) and the SK-MELB cell line (IC50 = 122 μM). The IC50 values are 30, 84,90, and 68 μM for Human SK-MEL-30, SK-MEL-2, SK-MEL-3,RPMI-7951 cells, respectively. 3,4-Dihydroxybenzylamine hydrobromide (1 μM-10 mM; 48 hours) cytotoxicity in SK-MEL-2 and SK-MEL-28 cell lines can be enhanced by application of buthionine sulfoximine (BSO). BSO treatment sensitizes marginally pigmented cells to the effects of 3,4-DHBA, and the presence of decreses IC50 values of 3,4-DHBA by 127% in the SK-MEL-28 cell line and by 148% in the SK-MEL-2 cell line. 3,4-Dihydroxybenzylamine hydrobromide exhibits IC50values of 10, 25, 67, and 184 μM for S91A, S91B, L1210, and SCC-25 cells, respectively[1]. |
| 體內(nèi)活性 | 3,4-Dihydroxybenzylamine hydrobromide (200, 400, 600 and 800 mg/kg; i.p.; 21 days) has different effects at different doses, the median life-span are 17, 24.5, 26, 29 and 25 days respectively. 3,4-Dihydroxybenzylamine hydrobromide (1000 mg/kg; i.p.; 7 days) has the least toxic effect in non-tumor-bearing B6D2F1 mice,and mice is tolerated at this dose[2]. |
| 存儲(chǔ)條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | DMSO : 80 mg/mL (363.54 mM), Sonication is recommended.
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| 關(guān)鍵字 | NSC-263475 Hydrobromide | Inhibitor | DNA polymerase | Tumor | NSC263475 | melanoma | NSC-263475 | DNA/RNA Synthesis | 3,4-Dihydroxybenzylamine | inhibit | 3,4Dihydroxybenzylamine hydrobromide | tyrosinase | NSC263475 Hydrobromide | NSC 263475 Hydrobromide | NSC 263475 | 3,4-Dihydroxybenzylamine Hydrobromide | 3,4 Dihydroxybenzylamine hydrobromide |
| 相關(guān)產(chǎn)品 | Acyclovir | 2,4-D | Guanidine hydrochloride | Folic acid | Resveratrol | 5-Fluorouracil | Temozolomide | Thymidine | Ribavirin | Rifampicin |
| 相關(guān)庫(kù) | 抑制劑庫(kù) | 經(jīng)典已知活性庫(kù) | 抗癌化合物庫(kù) | 已知活性化合物庫(kù) | 抗衰老化合物庫(kù) | DNA 損傷和修復(fù)分子庫(kù) |