化合物 Ciliobrevin A|T3156|TargetMol

價(jià)格	￥279	￥438	￥591
包裝	2mg	5mg	10mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-09-29

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產(chǎn)品詳情

中文名稱:化合物 Ciliobrevin A	英文名稱:Ciliobrevin A
CAS:302803-72-1	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.22%	產(chǎn)品類別: 抑制劑
貨號(hào): T3156

Product Introduction

Bioactivity

名稱	Ciliobrevin A
描述	Ciliobrevin A (HPI-4) is an inhibitor of hedgehog signaling pathway with an IC50 <10 μM.
細(xì)胞實(shí)驗(yàn)	Using MTS-8 assay to measure cell proliferation and the toxicity of this drug. 2000 cells were plated in 96-well plates per well. HPI-4 was added to cells at concentrations of 0, 5 and 10 μM in 100 μl DMEM/F12 with 10% FBS and incubated for 0, 1, 3, 6 and 9 days. Then, 10 μl MST-8 was added to the media in each well and incubated in an environment without light for 90 min. The absorbance value was measured using an enzyme microplate reader at 450 nm wavelength. The relative viability of cells was expressed by OD value.(Only for Reference)
激酶實(shí)驗(yàn)	Smo-binding assays are conducted with BODIPY-cyclopamine and Smo-overexpressing HEK 293T cells, using a CMVpromoter-based SV40 origin-containing expression construct for Smo-Myc3 (murine Smo containing three consecutive Myc epitopes at the C terminus). HEK 293T cells are seeded into eight-well chambered coverslips (80,000 cells/well) and cultured in DMEM containing 10% FBS, 100 U/mL penicillin, and 0.1 mg/mL streptomycin. The cells are cultured until they reached 55 to 65% confluency (14-18 h), after which they are transfected with the Smo-Myc3 expression construct and Transit-LT1. Twenty-four hours after transfection, the cells are washed with PBS and cultured in DMEM containing 0.5% FBS, 5 nM BODIPY-cyclopamine, and various concentrations of either cyclopamine or individual HPIs. After 30 min, 10 μM Hoescht 33342 is added to each well, and the HPIs are incubated with the cells for an additional 30 min. The cells are then washed two times with PBS buffer, once with phenol red-free DMEM containing 0.5% FBS, and immediately imaged using a DMI6000B compound microscope. Images are background-substracted using ImageJ software with a rolling ball size of 75 pixels, and BODIPY-cyclopamine intensity is then determined using Metamorph software. Circular regions with a diameter of 300 pixels are placed over regions containing uniformly confluent cells, and the pixel intensities of approximately 20 regions from four independent images is used to determine the average BODIPY-cyclopamine levels for each experimental condition[1].
體外活性	經(jīng)Ciliobrevin A培養(yǎng)的Shh-EGFPFLAG-Gli2細(xì)胞表現(xiàn)出原生纖毛的縮短，并且在一大部分經(jīng)Ciliobrevin A處理的細(xì)胞中，該細(xì)胞器缺失。Ciliobrevin A擾亂Shh-LIGHT2FLAG-Gli1細(xì)胞中原生纖毛的形成，并促進(jìn)FLAG-Gli1在此細(xì)胞器遠(yuǎn)端積累。Ciliobrevin A顯著抑制這些神經(jīng)前體細(xì)胞的增殖，通過組蛋白H3磷酸化水平(pH3)測(cè)量，并減少細(xì)胞內(nèi)cyclin D1蛋白以及Gli1、Gli2和N-Myc轉(zhuǎn)錄本的水平。Ciliobrevin A能夠阻止表達(dá)SmoM2的CGNPs的增殖，并應(yīng)對(duì)缺乏Su(fu)功能的CGNPs同樣有效，而Smo抑制劑Cyclopamine對(duì)這兩種致癌損傷均無效。Ciliobrevin A阻止了Shh刺激下FLAG-Gli2全長(zhǎng)/抑制劑比率的增加，但HPI-2和HPI-3無顯著影響。Ciliobrevin A增加了纖毛中FLAG-Gli2的水平，這種增加與其對(duì)總FLAG-Gli2水平的影響不成比例[1]。
存儲(chǔ)條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	DMSO : 65 mg/mL (181.47 mM), Sonication is recommended. Ethanol : 1 mg/mL (2.79 mM)
關(guān)鍵字	Hedgehog \| HPI 4 \| HPI4 \| inhibit \| Inhibitor \| Ciliobrevin A
相關(guān)產(chǎn)品	ALLO-2 \| Tolnaftate \| BMS-833923 \| SAG \| SMO-IN-2 \| Triparanol \| Ellagic acid \| Naftifine hydrochloride \| GANT 61 \| Vismodegib \| MK-4101 \| Itraconazole
相關(guān)庫	抑制劑庫 \| 經(jīng)典已知活性庫 \| 抗癌化合物庫 \| 已知活性化合物庫 \| 抗肥胖化合物庫 \| GPCR靶點(diǎn)分子庫 \| 膜蛋白靶向化合物庫 \| Wnt/Hedgehog/Notch 通路化合物庫 \| 神經(jīng)元分化化合物庫 \| 細(xì)胞重編程化合物庫

關(guān)鍵字： Hedgehog Pathway Inhibitor 4|||HPI-4|TargetMol

公司簡(jiǎn)介

TargetMol Chemicals Inc. 總部位于馬薩諸塞州波士頓，致力于為全球生化領(lǐng)域科學(xué)家的研究提供專業(yè)的產(chǎn)品和服務(wù)。TargetMol?品牌的客戶群分布于40多個(gè)國(guó)家和地區(qū)，已發(fā)展成為全球知名的化合物庫和小分子化合物研究供應(yīng)商。 TargetMol?可提供160多種滿足不同需求的化合物庫，以及多種類型的生化試劑產(chǎn)品，包括12000多種抑制劑、16000多種天然產(chǎn)物和各類多肽、抗體、生命科學(xué)試劑盒等，此外，我們還建設(shè)有CADD（計(jì)算機(jī)輔助藥物設(shè)計(jì)）研究中心、藥理實(shí)驗(yàn)室、藥化合成平臺(tái)三大技術(shù)中心，全方位滿足客戶的定制需求。憑借我們優(yōu)質(zhì)的產(chǎn)品和服務(wù)、快速高效的全球供應(yīng)鏈和專業(yè)的技術(shù)支持，我們將有效幫助您縮短研發(fā)周期，取得更成功的結(jié)果。

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