IBMX,3-異丁基-1-甲基黃嘌呤|T1713|TargetMol

價(jià)格	￥145	￥192	￥293
包裝	5mg	10mg	25mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱(chēng):IBMX,3-異丁基-1-甲基黃嘌呤	英文名稱(chēng):IBMX
CAS:28822-58-4	品牌: TargetMol
產(chǎn)地: 美國(guó)	保存條件: store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.86%	產(chǎn)品類(lèi)別: 抑制劑
貨號(hào): T1713

Product Introduction

Bioactivity

名稱(chēng)	IBMX
描述	IBMX (Methylisobutylxanthine) is a broad-spectrum phosphodiesterase (PDE) inhibitor with inhibitory activity against PDE3, PDE4, and PDE5 (IC50=6.5/26.3/31.7 μM). IBMX enhances the intracellular cAMP level.
細(xì)胞實(shí)驗(yàn)	Intracellular cyclic GMP and cyclic AMP concentrations in guinea-pig TSMCs were assayed as previously described. In brief, cells were grown in 24-well plates 10^5 cells per well. At confluence, monolayer cells were washed with phosphate buffer solution (PBS) and then incubated with KMUP-1 (0.1–100 μM) in the presence of 100 μM IBMX for 20 min. Incubation was terminated by the addition of 10% trichloroacetic acid (TCA). Cell suspensions were sonicated and then centrifuged at 2500 × g for 15 min at 4°C. To remove TCA, the supernatants were extracted three times with 5 volumes of water-saturated diethyl ether. Then, the supernatants were lyophilized and the cyclic GMP or AMP of each sample was determined by using commercially available radioimmunoassay kits [1].
動(dòng)物實(shí)驗(yàn)	Male mice (25-35 g), obtained from the animal house of Faculty of Medicine, were kept in controlled environmental conditions (temperature: 23±2 oC; light-dark cycle: 7 a.m. to 7 p.m.) and were divided randomly into groups of seven. All test compounds were dissolved in DMSO and diluted to desired concentration with less than 1% DMSO. For the experiment, the test compound (IBMX, milrinone, MCPIP, mc1, mc2, mc5 or mc6) or solvent (control) was injected subcutaneously to mice at 1 mg/kg dosage twice a day (8:00 a.m. and 8:00 p.m.) for 7 days. On day 8, animals were anesthetized with intraperitoneal injection of thiopental (80 mg/kg) and blood samples were obtained from their hearts and then the liver was dissected. Each sample was centrifuged for 5 min and its serum was separated [3].
體外活性	方法：從 HK 喂養(yǎng)的大鼠分離的 CCD 用 IBMX (100 μM) 預(yù)處理 20 min，并檢查 ANG II 或 cGMP 對(duì)通道活性的影響。結(jié)果：IBMX 激活了 ROMK 通道并阻止了 ANG II 進(jìn)一步激活通道。[1] 方法：豚鼠 TSMC 的原代培養(yǎng)物，在 IBMX (100 μM) 存在的情況下，檢測(cè) KMUP-1 對(duì) cAMP 和 cGMP 水平的影響。結(jié)果：IBMX 和 KMUP-1 顯著增加了 cAMP 和 cGMP 水平。單獨(dú)使用 KMUP-1 對(duì) cAMP 和 cGMP 水平與在有 IBMX 的條件下沒(méi)有顯著差異。[2] 方法：哺乳動(dòng)物細(xì)胞 CHO 用 IBMX (10-1000 μM) 處理，使用膜片鉗技術(shù)測(cè)量全細(xì)胞電流。結(jié)果：IBMX 對(duì) THIK-1 電流影響的穩(wěn)態(tài)劑量反應(yīng)曲線可以擬合為 Hill 系數(shù)為 1 和 IC50 為 120 μM。[3]
體內(nèi)活性	方法：為檢測(cè)對(duì)小鼠的代謝作用，將 IBMX (1 mg/kg) 皮下注射給小鼠，每天兩次，持續(xù)七天。結(jié)果：IBMX 顯著增加了小鼠的血糖水平（血糖，mg/dl，對(duì)照=141，IBMX=210）。[4] 方法：為檢測(cè)對(duì)高血糖小鼠的代謝作用，將葡萄糖 (0.5 g/kg) 和 IBMX (1 mg/kg) 股靜脈注射給 Wistar 大鼠。結(jié)果：在高血糖大鼠中，IBMX 能降低血糖，IBMX 不會(huì)改變血漿胰島素水平，IBMX 降低了肝糖原儲(chǔ)存。[4]
存儲(chǔ)條件	store at low temperature \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	H2O : 2.22 mg/mL (10 mM), Sonication is recommended. DMSO : 16.7 mg/mL (75 mM) 10% DMSO+90% Saline : 1.67 mg/mL (7.51 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. Ethanol : 22.24 mg/mL (100 mM), Sonication is recommended.
關(guān)鍵字	Phosphodiesterase (PDE) \| IBMX \| inhibit \| Inhibitor
相關(guān)產(chǎn)品	Icariin \| Vardenafil hydrochloride \| Apremilast \| Indomethacin \| Roflumilast \| Sildenafil citrate \| Theophylline \| Acefylline \| Theobromine \| Theophylline monohydrate \| Isoprenaline hydrochloride \| Doxofylline
相關(guān)庫(kù)	抑制劑庫(kù) \| 經(jīng)典已知活性庫(kù) \| 已知活性化合物庫(kù) \| 活性脂質(zhì)化合物庫(kù) \| 代謝化合物庫(kù) \| 抗阿爾茨海默癥化合物庫(kù) \| 抗心血管疾病化合物庫(kù) \| 神經(jīng)退行性疾病化合物庫(kù) \| 抗代謝疾病化合物庫(kù) \| 人代謝物化合物庫(kù)

關(guān)鍵字： Methylisobutylxanthine|||Isobutylmethylxanthine|||IBMX,3-異丁基-1-甲基黃嘌呤|||3-Isobutyl-1-methylxanthine|||1-Methyl-3-Isobutylxanthine|TargetMol

公司簡(jiǎn)介

上海陶術(shù)生物科技有限公司為美國(guó)Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國(guó)波士頓、德國(guó)慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專(zhuān)業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實(shí)體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷(xiāo)售到個(gè)性化定制合成；從對(duì)明確靶點(diǎn)的分子篩選到對(duì)明確分子的多靶點(diǎn)篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿(mǎn)足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過(guò)在中國(guó)市場(chǎng)五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過(guò)五百家學(xué)校和各類(lèi)企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊(cè)資本	566.2651萬(wàn)人民幣
員工人數(shù)	100-500人	年?duì)I業(yè)額	￥ 1億以上
主營(yíng)行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營(yíng)模式	貿(mào)易,試劑,定制,服務(wù)

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公司成立：12年
注冊(cè)資本：566.2651萬(wàn)人民幣
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主營(yíng)產(chǎn)品：小分子抑制劑，藥物篩選化合物庫(kù)，天然產(chǎn)物，活性分子化合物等
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