| 名稱 | Kaempferol |
| 描述 | Kaempferol (Robigenin) is a natural flavonoid and an inverse agonist of ERRα and ERRγ. Kaempferol has a wide range of antitumor, anti-inflammatory, antioxidant, antibacterial and antiviral activities. |
| 細(xì)胞實驗 | Kaempferol is prepared in DMSO (100 mM) and stored (-20°C), and then diluted with appropriate medium[2]. Ovarian cancer cells are seeded in 96-well plates at 2000 cells/well and incubated overnight before treatment with 0-160 μM Kaempferol for 24 hours in triplicates. The medium is removed, and the plates are freeze-thawed to lyse cells. Each well is added with 200 μL 1× CyQUANT cell lysis buffer containing 5x SYBR Green I and incubated at room temperature (RT) for 5 minutes. The reaction (50 μL) is transferred to PCR strip tubes and the fluorescent signal is measured at 90°C with a real-time Chromo4 PCR instrument. To ensure that cell proliferation assays are performed within a linear range of cell numbers, a standard curve is generated by seeding different amount of OVCAR-3 cells (based on counting with a hemacytometer) in a 96-well plate, and measuring genomic DNA abundance after overnight incubation. Three independent experiments are performed and data is pooled for statistical analysis[2]. |
| 激酶實驗 | Right atria or sinus nodal cells are homogenized in lysis buffer consisting of (50 mM Tris-HCl pH 7.5, 100 mM KCl, 1 mM ethylenediamine tetraacetic acid, 1 mM ethylene glycol tetraacetic acid, 1 mM dithiothreitol, 0.1 mM phenylmethylsulfonyl fluoride, 0.5 mM Benzamidine, 20 mg/L Leupeptin, 20 mM sodium pyrophosphate, 50 mM NaF, and 50 mM sodium β-glycerophosphate), and total protein content is determined by the Bradford assay. Caspase-3 activity is determined by EnzChek Caspase-3 Assay Kit[3]. |
| 體外活性 | 方法：人乳腺癌細(xì)胞 MDA-MB-231 用 Kaempferol (0.01-100 μM) 處理 72 h，使用 MST assay 檢測細(xì)胞活力。
結(jié)果：Kaempferol 以劑量依賴的方式抑制細(xì)胞增殖，IC50 值為 43 μmol/L。[1]
方法：人胃癌細(xì)胞 AGS 和 SNU638 用 Kaempferol (50 μM) 處理 8-24 h，使用 Western Blot 方法檢測靶點蛋白表達(dá)水平。
結(jié)果：Kaempferol 以時間依賴的方式增加 cleaved caspase-3 和 -9 并降低 Bcl-2 水平。[2] |
| 體內(nèi)活性 | 方法：為研究對失血性休克小鼠的影響，將 Kaempferol (10 mg/kg) 腹腔注射給經(jīng)歷失血性休克的 C57/BL6 的小鼠。
結(jié)果：Kaempferol 預(yù)處理失血性休克小鼠可顯著降低血漿 TNF-α 和 IL-6 水平；恢復(fù)了心、肺和肝的 MPO、SOD 和 MDA；并且增加了 HO-1 在相同器官中的表達(dá)。[3]
方法：為研究抗神經(jīng)炎癥作用，將 Kaempferol (25-100 mg/kg) 灌胃給藥給 BALB/c 小鼠，每天一次，持續(xù)七天，第七天注射 LPS (5 mg/kg)。
結(jié)果：Kaempferol 可能是一種很有前途的神經(jīng)保護(hù)劑，通過抑制 HMGB1 的釋放和下調(diào) TLR4/MyD88 炎癥途徑來緩解炎癥反應(yīng)和血腦屏障功能障礙。[4] |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2.86 mg/mL (9.99 mM), Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : 28.6 mg/mL (100 mM)
DMSO : 45 mg/mL (157.21 mM)
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| 關(guān)鍵字 | Kaempferol | Parasite | inhibit | Autophagy | Endogenous Metabolite | Apoptosis | Estrogen Receptor/ERR | Mitophagy | Human immunodeficiency virus | HIV | Mitochondrial Autophagy | Inhibitor |
| 相關(guān)產(chǎn)品 | Daidzein | Glycerol | Emtricitabine | Doxycycline | Sucrose | Hydroxychloroquine | Ferulic Acid | Lamivudine | Dextran sulfate sodium salt (MW 4500-5500) | 3-Indoleacetic acid | Thymidine | Paeonol |
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