鵝去氧膽酸|T0847

價格	￥190
包裝	20mg

最小起訂量	1mg
發(fā)貨地	上海
更新日期	2024-12-02

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產(chǎn)品詳情

中文名稱:鵝去氧膽酸	英文名稱:Chenodeoxycholic acid
CAS:474-25-9	品牌: TargetMol
產(chǎn)地: 美國	保存條件: Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
純度規(guī)格: 99.97%	產(chǎn)品類別: 抑制劑
貨號: T0847

Product Introduction

Bioactivity

名稱	Chenodeoxycholic acid
描述	Chenodeoxycholic acid (CDCA) is a bile acid, usually conjugated with either glycine or taurine. It acts as a detergent to solubilize fats for intestinal absorption and is reabsorbed by the small intestine. It is used as cholagogue, a choleretic laxative, and to prevent or dissolve gallstones.
細(xì)胞實驗	The cell viability is analyzed by incubating transfected HEK-293 cells and CHO cells for 1 h with the corresponding concentration of bile acid and staining with trypan blue. The toxicity of bile acids is analyzed using the tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) according to the cell proliferation kit I. No significant differences between control and bile acid-treated cells are obtained in both tests.
激酶實驗	Briefly, transfected HEK-293 cells, incubated in charcoal-treated Dulbecco's modified Eagle's medium for 24 h, are washed once with Hanks' solution and resuspended in a buffer containing 100 mM NaCl, 1 mM MgCl2, 1 mM EDTA, 1 mM EGTA, 250 mMsucrose, 20 mM Tris-HCl, pH 7.4. Cells are lysed by freezing in liquid nitrogen. Dehydrogenase activity is measured in a final volume of 20 μL containing the appropriate concentration of bile acid, 30 nCi of [3H]cortisol, and unlabeled cortisol to a final concentrations of 50 nM. The reaction is started by mixing cell lysate with the reaction mixture. Alternatively, endoplasmic reticulum microsomes are prepared from transfected HEK-293 cells and incubated with reaction mixture containing various concentrations of cortisol and CDCA. Incubation proceeded for 20 min, and the conversion of cortisol to cortisone is determined by thin layer chromatography (TLC). Because of the inaccuracy of the TLC method at low conversion rates and the end-product inhibition of 11βHSD2 at conversion rates higher than 60-70%, only conversion rates between 10 and 60% are considered for calculation. The inhibitory constant IC50 is evaluated using the curve-fitting program. Results are expressed as means±S.E. and consist of at least four independent measurements.
體外活性	Chenodeoxycholic acid （CDCA）和脫氧膽酸（DCA）均可抑制11 beta HSD2，其IC(50)值分別為22 mM和38 mM。Chenodeoxycholic acid 誘導(dǎo)皮質(zhì)醇依賴性核內(nèi)轉(zhuǎn)位，增加礦物皮質(zhì)激素受體（MR）的轉(zhuǎn)錄活性。[1] Chenodeoxycholic acid 通過激活膜G蛋白偶聯(lián)受體（TGR5）依賴性通路，顯著促進(jìn)Ishikawa細(xì)胞生長，增加Cyclin D1蛋白和mRNA表達(dá)。[2] 在培養(yǎng)的人類肝母細(xì)胞瘤細(xì)胞系Hep G2中，Chenodeoxycholic acid 可使LDL受體mRNA水平提高近4倍，HMG-CoA還原酶和HMG-CoA合酶mRNA水平提高2倍。[3] Chenodeoxycholic acid 誘導(dǎo)的Isc被Bumetanide、BaCl2和囊性纖維化跨膜導(dǎo)電調(diào)節(jié)器（CFTR）抑制劑CFTRinh-172抑制（≥67%）。Chenodeoxycholic acid 激活的Isc被腺苷酸環(huán)化酶抑制劑MDL12330A降低43%，Chenodeoxycholic acid 增加細(xì)胞內(nèi)cAMP濃度。[4] Chenodeoxycholic acid 通過增加其磷酸化、核內(nèi)積聚來激活C/EBPβ。Chenodeoxycholic acid 增強了含有C/EBP響應(yīng)元素（pGL-1651）的-1.65-kb GSTA2啟動子結(jié)構(gòu)的熒光素酶基因轉(zhuǎn)錄。Chenodeoxycholic acid 激活A(yù)MP激活的蛋白激酶（AMPK），促進(jìn)細(xì)胞外信號調(diào)節(jié)激酶1/2（ERK1/2）的激活。[5]
存儲條件	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
溶解度	Ethanol : 79 mg/mL (201.23 mM) DMSO : 40 mg/mL (101.89 mM), Sonication is recommended. H2O : < 1 mg/mL (insoluble or slightly soluble)
關(guān)鍵字	NR1H4 \| Endogenous Metabolite \| Inhibitor \| Chenodeoxycholic acid \| inhibit \| Autophagy \| FXR
相關(guān)產(chǎn)品	Guanidine hydrochloride \| Naringin \| Daidzein \| Glycerol \| Sucrose \| Hydroxychloroquine \| Ferulic Acid \| 3-Indoleacetic acid \| Stavudine \| Thymidine \| Paeonol \| Fumaric acid
相關(guān)庫	經(jīng)典已知活性庫 \| 抗癌上市藥物庫 \| EMA 上市藥物庫 \| 疼痛相關(guān)化合物庫 \| 高通量篩選天然產(chǎn)物庫 \| 抗癌臨床化合物庫 \| 抗癌藥物庫

關(guān)鍵字：鵝去氧膽酸|||Chenodiol|||CDCA|TargetMol

公司簡介

上海陶術(shù)生物科技有限公司為美國Target Molecule Corp. ( Target Mol ) 在上海建立的全資子公司。我們與美國波士頓、德國慕尼黑的同事一起，為北美、歐洲和亞洲從事藥物研發(fā)和生物學(xué)研究的科學(xué)家提供優(yōu)質(zhì)的產(chǎn)品和專業(yè)的服務(wù)。公司下設(shè)篩選事業(yè)部，化學(xué)事業(yè)部，生物事業(yè)部和新材料部。從虛擬篩選到實體化合物分子供應(yīng)；從商業(yè)化產(chǎn)品銷售到個性化定制合成；從對明確靶點的分子篩選到對明確分子的多靶點篩選，從高通量篩選到化學(xué)結(jié)構(gòu)優(yōu)化，我們都可以滿足您的科研用品及技術(shù)服務(wù)的需求。經(jīng)過在中國市場五年的精心耕耘，我們已成為篩選化合物領(lǐng)域優(yōu)秀的供應(yīng)商，為超過五百家學(xué)校和各類企業(yè)提供了品質(zhì)卓越的小分子化合物和藥物篩

成立日期	2013-04-18 （12年）	注冊資本	566.2651萬人民幣
員工人數(shù)	100-500人	年營業(yè)額	￥ 1億以上
主營行業(yè)	化學(xué)試劑,生物活性小分子	經(jīng)營模式	貿(mào)易,試劑,定制,服務(wù)

TargetMol中國（陶術(shù)生物）

VIP 12年

公司成立：12年
注冊資本：566.2651萬人民幣
企業(yè)類型：有限責(zé)任公司(自然人投資或控股)
主營產(chǎn)品：小分子抑制劑，藥物篩選化合物庫，天然產(chǎn)物，活性分子化合物等
公司地址：上海市閘北區(qū)江場三路28號4樓

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