| 名稱 | Venetoclax |
| 描述 | Venetoclax (ABT-199) is a Bcl-2 inhibitor (Ki<0.01 nM) with potent, selective, and orally active properties. Venetoclax has a 3-order-of-magnitude lower affinity for Bcl-xL and Bcl-W (Kis=48/245 nM). Venetoclax induces autophagy and apoptosis. |
| 細胞實驗 | RS4;11 cells were seeded at 50,000 per well in 96-well plates and treated with compounds diluted in half-log steps starting at 1 μM and ending at 0.00005 μM. All other leukemia and lymphoma cell lines were seeded at 15,000–20,000 cells per well in the appropriate medium and incubated with ABT-199 or navitoclax for 48 h. Effects on proliferation were determined using Cell TiterGlo reagent. EC50 values were determined by nonlinear regression analysis of the concentration-response data. Mouse FL5.12–BCL-2 and FL5.12–BCL-XL cells were propagated and assessed as described previously. Bak?/? Bax?/? double knockout mouse embryonic fibroblasts were seeded into 96-well microtiter plates at 5,000 cells per well in DMEM supplemented with 10% FBS. ABT-199 in the same culture medium was added in half-log dilutions starting at 5 μM. The cells were then incubated at 37 °C (5% CO2) for 48 h, and the effects on proliferation were determined using Cell TiterGlo reagent according to the manufacturer's instructions [1]. |
| 激酶實驗 | The equilibrium binding experiments of fluorescent peptides to Bcl-xL protein were performed in an Analyst 96-well plate reader under the following conditions: each individual well in a 96-well assay plate contained 5 μl DMSO, 15 nM fluorescent peptide, and increasing concentrations (from 0 to 2.24 μM) of Bcl-xL protein in assay buffer in a final volume of 125 μl. The plate was mixed on a shaker for 1 min and incubated at room temperature for an additional 15 min. The polarization in millipolarization units (mP) was measured at room temperature with an excitation wavelength at 485 nm and an emission wavelength at 530 nm. For assay stability testing, a plate containing a binding experiment was measured at different times over a 24-h period. Between each reading, the plate was covered with parafilm to prevent any solution evaporation. To determine the effect of DMSO on the assay, binding experiments were performed under conditions similar to those described above except that the amount of DMSO was varied from 0 to 4 to 8%. All experimental data were analyzed using Prism 3.0 software and Kd values were generated by fitting the experimental data using a sigmoidal dose-response nonlinear regression model [1]. |
| 動物實驗 | Female C.B-17 SCID mice (DoHH2 and Granta-519 xenografts) and female C.B-17 SCID-beige mice (RS4;11 and Toledo xenografts) were inoculated with 1 × 10^6 (DoHH2) or 5 × 10^6 (Granta-519, Toledo and RS4;11) cells subcutaneously in the right flank. The inoculation volume (0.2 ml) comprised a 50:50 mixture of cells in growth media and Matrigel. Electronic calipers were used to measure the length and width of each tumor 2–3 times per week. Tumor volume was estimated by applying the following equation: volume = length × width2/2. When tumors reached approximately 220 mm3, mice were size matched (day 0) into treatment and control groups. All xenograft trials were conducted using ten mice per group, and all mice were ear tagged and monitored individually throughout the studies. ABT-199 was formulated for oral dosing in 60% phosal 50 propylene glycol (PG), 30% polyethylene glycol (PEG) 400 and 10% ethanol, and bendamustine and rituximab were formulated in accordance with the manufacturer's instructions. ABT-199 was delivered approximately 2 h before bendamustine or bendamustine plus rituximab. TGImax was calculated as the greatest treatment response using the following equation: TGImax = (1 ? mean tumor volume of the treated group/mean tumor volume of the vehicle control group) × 100. The TGD (%) was determined as the percentage increase of the median time period for the treatment group to reach an arbitrary tumor volume of 1,000 mm3 relative to the vehicle control group. A complete tumor regression response was the portion of the population with tumors ≤25 mm3 for at least three consecutive measurements [1]. |
| 體外活性 | 方法：11 種人 T 細胞急性淋巴細胞白血病細胞 T-ALL 用 Venetoclax (0-12 μM) 處理 48 h，使用 Celltiter-Glo Luminescent Cell Viability Assay 檢測細胞活力。
結(jié)果：Venetoclax 對 11 種 T-ALL 的 IC50 值范圍為 0.2-10 μM。[1]
方法：人急性淋巴白血病細胞 RS4;11 用 Venetoclax (0.01-5 μM) 孵育 3.5 h，使用 Caspase-GLO kit 評估 Caspase-3/7 活性。
結(jié)果： Venetoclax 誘導(dǎo) Caspase 的激活，這是細胞凋亡特征之一。[2]
方法：人原代 HCL 白血病細胞用 Venetoclax (0.1-1 μM) 處理 24 h，使用 Flow Cytometry 方法檢測細胞死亡情況。
結(jié)果：Venetoclax 以劑量依賴的方式顯著增加了 HCL 細胞的細胞死亡。[3] |
| 體內(nèi)活性 | 方法：為檢測體內(nèi)抗腫瘤活性，將 Venetoclax (100 mg/kg in 60% PG+30% PEG 400+10% ethanol) 口服給藥給攜帶人彌漫大B細胞淋巴瘤 Toledo 的 C.B-17 SCID-beige 小鼠，每天一次，持續(xù)二十一天。
結(jié)果：Venetoclax 可顯著抑制 Toledo 腫瘤的生長 (TGImax=93%，TGD=220%)。[2]
方法：為檢測體內(nèi)抗腫瘤活性，將 Venetoclax (50 mg/kg in 10% ethanol+30% PEG 400+60% Phosal 50PG，口服給藥，每天一次) 和 anti-PD-1 (10 mg/kg in PBS，腹腔注射，每四天三次) 給藥給攜帶小鼠結(jié)直腸癌腫瘤 MC38 的 C57BL/6 小鼠，持續(xù)十四天。
結(jié)果：Venetoclax 可以增強免疫檢查點抑制劑 (ICIs) 的抗腫瘤功效，同時增加 PD-1+T 效應(yīng)記憶細胞。Venetoclax 在體外對抗原刺激的反應(yīng)中不會損害人類 T 細胞的功能，也不會拮抗 anti-PD-1 誘導(dǎo)的 T 細胞活化。[4] |
| 存儲條件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| 溶解度 | H2O : < 1 mg/mL (insoluble or slightly soluble)
Ethanol : < 1 mg/mL (insoluble or slightly soluble)
DMSO : 100 mg/mL (115.15 mM)
|
| 關(guān)鍵字 | bioavailable | GDC0199 | orally | inhibit | Inhibitor | Bcl-2 Family | GDC 0199 | Venetoclax | Autophagy |
| 相關(guān)產(chǎn)品 | Oxyresveratrol | Guanidine hydrochloride | Naringin | Taurine | Gefitinib | Hydroxychloroquine | Curcumin | Stavudine | Paeonol | Sodium 4-phenylbutyrate |
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