Human LRRC15 / LIB Protein, His Tag (MALS verified)

分子別名（Synonym）

Leucine-rich repeat-containing protein 15,LRRC15,LIB,hLib

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human LRRC15, His Tag (LR5-H52H3) is expressed from human 293 cells (HEK293). It contains AA Tyr 22 - Gly 538 (Accession # Q8TF66-1).

Predicted N-terminus: Tyr 22

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 59.8 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

LRRC15（富含亮氨酸重復(fù)序列的蛋白質(zhì)15）也被稱為LIB和hLib。LRRC15在多種實(shí)體瘤中高度表達(dá)。LRRC15在許多實(shí)體瘤（如乳腺、頭頸部、肺、胰腺）的基質(zhì)成纖維細(xì)胞上表達(dá)，也直接在間充質(zhì)來源的癌癥細(xì)胞亞群（如肉瘤、黑色素瘤、膠質(zhì)母細(xì)胞瘤）上表達(dá)。TGFβ誘導(dǎo)活化的成纖維細(xì)胞（α-SMA+）和間充質(zhì)干細(xì)胞表達(dá)LRRC15。這些集體發(fā)現(xiàn)表明，LRRC15是一種新型的CAF和間充質(zhì)標(biāo)志物，可作為治療LRRC15陽性間質(zhì)促纖維增生癥或間充質(zhì)來源癌癥的治療靶點(diǎn)。ABBV-085是一種針對(duì)LRRC15的含單甲基金抑制素E（MMAE）的抗體-藥物偶聯(lián)物（ADC），它作為單一療法或與標(biāo)準(zhǔn)護(hù)理療法相結(jié)合，對(duì)LRRC15中的異常陽性/癌癥陰性和LRRC15癌癥陽性模型表現(xiàn)出強(qiáng)大的臨床前療效。