Human CD20 / MS4A1 Full Length Protein, His Tag (Detergent) (SPR verified)

分子別名（Synonym）

MS4A1,CD20,MS4A-1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human CD20 Full Length Protein, His Tag (CD0-H52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).

Predicted N-terminus: Met 1

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 35.2 kDa. The protein migrates as 40 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>85% as determined by SDS-PAGE.

制劑（Formulation）

This product is not suitable for cell based experiments due to cytotoxicity of DDM.

DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumastance should you remove DDM and CHS.

DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible to your application, remove glycerol just before immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

運(yùn)輸（Shipping）

This product is supplied and shipped with dry ice, please inquire the shipping cost.

存儲（Storage）

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

The product MUST be stored at -70°C or lower upon receipt;

-70°C for 3 months under sterile conditions.

背景（Background）

B淋巴細(xì)胞抗原CD20也稱為B淋巴細(xì)胞表面抗原B1、白細(xì)胞表面抗原Leu-16、跨膜4結(jié)構(gòu)域亞家族A成員1和MS4A1，是一種活化的糖基化磷蛋白，從B前相（CD45R+、CD117+）開始在所有B細(xì)胞表面表達(dá)，濃度逐漸增加，直到成熟。CD20在B細(xì)胞發(fā)育的所有階段都有表達(dá)，除了第一個和最后一個階段；它從晚期pro-B細(xì)胞通過記憶細(xì)胞存在，但不存在于早期pro-B細(xì)胞或漿母細(xì)胞和漿細(xì)胞上。在B細(xì)胞淋巴瘤、毛細(xì)胞白血病、B細(xì)胞慢性淋巴細(xì)胞白血病和黑色素瘤癌癥干細(xì)胞中發(fā)現(xiàn)。該蛋白沒有已知的天然配體，其功能是實(shí)現(xiàn)最佳的B細(xì)胞免疫反應(yīng)，特別是針對T非依賴性抗原。懷疑它在細(xì)胞膜中充當(dāng)鈣通道。CD20/MS4A1是單克隆抗體（mAb）利妥昔單抗、伊維單抗和托西莫單抗的靶點(diǎn)，這些抗體都是治療所有B細(xì)胞淋巴瘤和白血病的活性藥物。CD20/MS4A1的缺陷是免疫缺陷共同變量5型（CVID5）的原因；也稱為由于CD20缺陷引起的抗體缺乏癥。CVID5是一種原發(fā)性免疫缺陷，其特征是抗體缺乏、低丙種球蛋白血癥、反復(fù)細(xì)菌感染和無法對抗原產(chǎn)生抗體反應(yīng)。