Human DLL3 Protein, His Tag (MALS verified)

分子別名（Synonym）

Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human DLL3, His Tag (DL3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Leu 492 (Accession # Q9NYJ7-1).

Predicted N-terminus: Ala 27

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 50.4 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS with Arginine, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

Delta樣蛋白3（DLL3）是一種跨膜蛋白，屬于Notch配體的Delta/Serrate/Lag-2（DSL）家族。可能需要沿著特定的分化途徑轉(zhuǎn)移神經(jīng)元。在近軸中胚層的分割過程中，在體節(jié)邊界的形成中起作用。DLL3蛋白在腫瘤細(xì)胞表面表達(dá)，但在正常成人組織中不表達(dá)。