IRDye 700 AP-1 Consensus Oligonucleotide

IRDye 700 CREB Consensus Oligonucleotide

IRDye 700 HIF-1 Consensus Oligonucleotide

IRDye 700 p53 Consensus Oligonucleotide

IRDye 700 STAT3 Consensus Oligonucleotide

IRDye 700 Sp-1 Consensus Oligonucleotide

IRDye 700 NF?B Consensus Oligonucleotide

IRDye 700 AP-1 共有寡核苷酸

5' -- CGC TTG ATG ACT CAG CCG GAA -- 3'

3' -- GCG AAC TAC TGA GTC GGC CTT -- 5'

帶下劃線的核苷酸是結(jié)合位點(diǎn)。 IRDye 700 寡核苷酸以 25 μL 的 50 nM（或 50 fmol/μL）雙鏈 DNA 形式提供。

AP-1 是一種轉(zhuǎn)錄因子，可調(diào)節(jié)基因表達(dá)并控制許多細(xì)胞過程，包括分化、增殖和凋亡。 出于這個(gè)原因，它是 EMSA 中用于評估蛋白質(zhì)結(jié)合的常見寡核苷酸。

由于 IRDye 紅外染料對光有些敏感，因此在孵育期間將結(jié)合反應(yīng)保持在黑暗中（例如，將試管放入抽屜或簡單地用鋁箔蓋住裝有試管的架子）。 孵育期后，將 10X Orange Loading Dye (P/N 927-10100) 添加到結(jié)合反應(yīng)中進(jìn)行電泳。

5′ -- CGC TTG ATG ACT CAG CCG GAA -- 3′

3′ -- GCG AAC TAC TGA GTC GGC CTT -- 5′

Underlined nucleotides are the binding site. IRDye 700 oligonucleotides are supplied as 25 μL of 50 nM (or 50 fmol/μL) double-stranded DNA.

AP-1 is a transcription factor that regulates gene expression and controls a number of cellular processes including differentiation, proliferation, and apoptosis. For this reason, it is a common oligo used in EMSA to assess protein binding.

Since IRDye infrared dyes are somewhat sensitive to light, it is best to keep binding reactions in the dark during incubation periods (e.g., put the tubes into a drawer or simply cover the rack containing tubes with aluminum foil). After the incubation period, 10X Orange Loading Dye (P/N 927-10100) is added to the binding reaction for electrophoresis.