IRDye 680RD Streptavidin
IRDye 680RD Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 μg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
For membrane-based applications and In-Gel Westerns, it is recommended to add SDS (0.02% to 0.1% final concentration), in addition to Tween? 20 (0.1 to 0.2% final concentration) during the detection incubation step to reduce non-specific background staining.
IRDye 680RD 鏈霉親和素
IRDye 680RD 鏈霉親和素以液體形式提供，緩沖液中含有 10 mM 磷酸鹽、183 mM NaCl、2.7 nM KCl，pH 7.4，疊氮化鈉 0.005% (w/v) 作為防腐劑。
要使用，請(qǐng)?jiān)谑褂们岸虝弘x心以消可能在溶液中形成的聚集體。 這將減少非特異性背景染色。 對(duì)于大多數(shù)應(yīng)用，0.2 到 1.0 μg/ml（1:1,000 到 1:5,000）的濃度通常是令人滿意的； 但是，可能需要根據(jù)經(jīng)驗(yàn)確定適當(dāng)?shù)南♂尪取?/p>
對(duì)于基于膜的應(yīng)用和 In-Gel Westerns，建議在檢測(cè)孵育步驟中添加 SDS（0.02% 至 0.1% 濃度）和 Tween? 20（0.1 至 0.2% 濃度）以減少非 特定背景染色。
關(guān)鍵字： IRDye 680RD;鏈霉親和素;
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