絲氨酸肽酶抑制因子Kazal型5(SPINK5)重組蛋白
[ PROPERTIES ]
Residues: Gly699~Ser976 (Accession # Q9NQ38),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 35.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.
MGSS HH HHH H SSG LVP RGS H MASMTG GQ QM GRGSEF -G G GNTQDE CA EYREQMK NG R LS CT RE S DPV RD ADGKSY N NQ C TM CKA K LE RE AE RK NEYS RSRS NG TGSESG KDT CDEF RS Q MK N GK LI CT R ES DPV RGP DG KT HG NK C TMCKEKLEREAAE KKKKEDEDRS NTGERSNTGE RSNDKEDLCR EFRSMQRNGK LICTRENNPVRGPYGKMHIN KCAMCQSIFD REANERKKKD EEKSSSKPSN NAKDECSEFRNYIRNNELICPRENDPVHGA DGKFYTNKCY MCRAVFLTEA LERAKLQEKP SHVRAS
[ REFERENCES ]
1.Tartaglia-Polcini A., et al. (2006) J. Invest. Dermatol. 126:315-324.
2. Sprecher E., et al. (2001) J. Invest. Dermatol. 117:179-187.
3. Ahmed A., et al. (2001) J. Protein Chem. 20:273-278.
4. Deraison C., et al. (2007) Mol. Biol. Cell 18:3607-3619.
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關鍵字： PCTT;LETKI;NETS;絲氨酸肽酶抑制因子Kazal型5(SPI;
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