中文名稱

血漿/血清循環(huán)和核外RNA純化試劑盒（提取試劑盒）

英文名字

Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) (50)

供應(yīng)商

Norgen Biotek

產(chǎn)品貨號

NGB-42800

產(chǎn)品報價

￥7812/50Preps

產(chǎn)品說明書

點(diǎn)擊查看

購買方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868。

產(chǎn)品新聞

背景資料

血漿/血清循環(huán)和核外RNA純化試劑盒（提取試劑盒）產(chǎn)品的背景資料詳情查看Norgen Biotek品牌提供的產(chǎn)品說明書

產(chǎn)品描述

Norgen’s Plasma/Serum Circulating and Exosomal RNA Purification Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating RNA and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 5 mL. This kit is able to isolate all sizes of circulating and exosmal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using this kit. This kit is suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.Preparation time for a single sample is less than 40 minutes. The purified plasma/serum free-circulating and exosmal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.Free-circulating plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating RNAs have the potential to provide biomarkers for other disease states. Free-circulating RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.

產(chǎn)品特點(diǎn)

Isolate all sizes of circulating and exosomal RNA, including microRNA; Versatile plasma and serum input volumes (0.25 mL to 5 mL); Concentrate circulating and exosomal RNA into small elution volumes; Isolate inhibitor-free circulating RNA; Bind and elute all RNA irrespective of size or GC content, without bias.

保存建議

所有緩沖液應(yīng)密封保存，并在室溫（15-25°C）下保存長達(dá)1年。具體保存情況收到產(chǎn)品后請按說明書存放。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術(shù)公司，基于專利的碳化硅膜純化技術(shù)和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設(shè)備，包括用于體外診斷領(lǐng)域）和ISO 15189（可用于臨床檢測機(jī)分子診斷領(lǐng)域的研發(fā)能力）認(rèn)證的生物科技公司。 目前該公司的產(chǎn)品多達(dá)700余種，提供一流的樣品制備試劑盒，應(yīng)用于基因組和蛋白組等不同的領(lǐng)域。其產(chǎn)品主要覆蓋包括各種標(biāo)本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。