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??????????II

??????????II
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9025-64-3
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??????????II
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??????????II
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DEOXYRIBONUCLEASE II
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DNASE II;DNASE-11;EC 3.1.4.6;EC 3.1.22.1;IUB: 3.1.22.1;DNASE II TYPE V;DNASE II TYPE IV;DEOXYRIBONUCLEASE II;Nuclease, deoxyribo-, II;DEOXYRIBONUCLEASE II TYPE V
CBNumber:
CB7111750
???:
NULL
??? ??:
0
MOL ??:
Mol file

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-20°C
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White to light yellow
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bovine spleen
Specific Activity
2,000-6,000Kunitz units/mg protein (biuret)
EPA
Nuclease, deoxyribo-, II (9025-64-3)

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WGK ?? 3
F ?????? 10-21
TSCA Yes

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Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples
  • to digest retinal explants for single-cell suspension preparation
  • for isolation of stromal cells from human colon tissue
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.

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