L-イソロイシン 化學(xué)特性,用途語(yǔ),生産方法
外観
白色～ほとんど白色粉末～結(jié)晶
定義
本品は、次の化學(xué)式で表されるアミノ酸である。
解説
L-イソロイシン，2-amino-3-methylvaleric acid．C6H13NO2(131.18)．略稱IleまたはI．種々のタンパク質(zhì)，ペプチドに構(gòu)成アミノ酸として含まれている．必須アミノ酸の一つ．大豆ミール，甜(てん)菜糖廃液，カゼインなどの加水分解液より等電點(diǎn)沈殿法，アゾベンゼン-p-スルホン酸塩法で分離する．発酵法によっても生産される．"不斉炭素原子を2個(gè)含むので，二つのラセミ體が存在する．L-イソロイシンの熱分解によって得られる光學(xué)活性アミルアミンは，発酵アミルアルコールから導(dǎo)かれるものと同じであるから，これらのβ位の立體配置は等しいことがわかる．D,L-イソロイシンは2-ブロモ-3-メチル吉草酸から種々の方法により合成できる．L-イソロシンは，斜方晶りん片狀結(jié)晶で苦味をもつ．融點(diǎn)284 ℃．[α]D20"＋11.29°(水)．pK1 2.36，pK2 9.68．pI6.02．水に難溶，熱エタノールに微溶，他の有機(jī)溶媒に不溶．[CAS 73-32-5]
森北出版「化學(xué)辭典（第2版）
化粧品の成分用途
ヘアコンディショニング剤、皮膚コンディショニング剤
効能
イソロイシン補(bǔ)充薬
説明
Crystalline leaflets or a white, crystalline powder having a bitter
taste. It is soluble in 25 parts of water, slightly soluble in hot
alcohol, and soluble in diluted mineral acids and in alkaline solutions. It sublimes at between 168°C and 170°C, and melts with
decomposition at about 284°C. The pH of a 1 in 100 solution is
between 5.5 and 7.0.
Absolute: The absolute is prepared by alcoholic extraction of the
concrete in approximately 55 to 60% yields. The absolute consists
of a semisolid, olive-green mass with a characteristic ambergris
odor.
Resinoid and Resin Absolute: The resinoid consists of the hydro carbon extract of the crude resin. The resin absolute consists of
the alcoholic extract of the crude labdanum resin. Since this prod uct is a semisolid, nonpourable mass, a high-boiling, odorless
solvent may be added prior to evaporation of the alcohol to prepare
a pourable liquid.
化學(xué)的特性
White crystalline powder
使用
L-Isoleucine, also known as Isoleucine, is an amino acid that is an isomer of leucine. It is important in hemoglobin synthesis and regulation of blood sugar and energy levels.
L-Isoleucine is one of the essential amino acids that cannot be made by the body and is known for its ability to help endurance and assist in the repair and rebuilding of muscle. This amino acid is important to body builders as it helps boost energy and helps the body recover from training.
L-Isoleucine is also classified as a branched-chain amino acid (BCAA). There are three branched-chain amino acids in the body, with the others being L-Valine, and L-Leucine, and all of them help promote muscle recovery after exercise. Isoleucine is actually broken down for energy within the muscle tissue.
調(diào)製方法
An advantageous fermentation method for production of L-isoleucine is the use of chemically synthesized substrates that only require a few steps to be converted to L-isoleucine. Among these the natural precursors 2-ketobutyrate or D,L-2-hydroxybutyrate have been used for the production with Corynebacterium glutamicum. A leucine requiring mutant of Corynebacterium glutamicum, with increased D-lactate utilization and consuming D,L-2-hydroxybutyrate, accumulates 13.4 g/L L-isoleucine. However, exploitation of this process is hampered by formation of byproducts. Sugar based L-isoleucine processes have been developed with strains of Corynebacterium glutamicum Serratia marcescens, and Escherichia coli. The mutant Escherichia coli H- 8285, being resistant to thiaisoleucine, arginine hydroxamate, and D,L-ethionine accumulates 26 g/L L-isoleucine in 45 h in a fed-batch process. Introduction of resistance to 6-dimethylaminopurine in strain H-8285 resulted in a mutant Escherichia coli H-8461 that increased L-isoleucine accumulation to 30.2 g/L. The biosynthesis of L-isoleucine has been investigated in detail on the level of involved genes, thus recombinant strains are being constructed with high productivity and selectivity. An appropriate balance of homoserine dehydrogenase and threonine dehydratase activities in the construct Corynebacterium glutamicum DR17/pECM3::ilvA (V323A) with feedback-resistant aspartate kinase create a specific productivity of 0.052 g L-isoleucine per gram dry biomass per hour. The recombinant strain Escherichia coli AJ13100 produces L-isoleucine from glucose with high selectivity in 30% yield.
定義
ChEBI: The L-enantiomer of isoleucine.
副作用
L-Isoleucine is generally considered a safe ingredient. The side effects may: Intake of Isoleucine can lead to nausea. Large doses can result in a stomach upset and indigestion.
概要
必須アミノ酸の一種
純化方法
Crystallise L-isoleucine from H2O by addition of 4 volumes of EtOH or from aqueous MeOH. It sublimes at 170-181o/0.3mm with 99.7% recovery, unracemised [Gross & Gradsky J Am Chem Soc 77 1678 1955]. [Greenstein & Winitz The Chemistry of the Amino Acids J. Wiley, Vol 1 p 183-191, Vol 3 pp 2043-2073 1961, Huffman & Ingersoll J Am Chem Soc 73 3366 1951, Beilstein 4 IV 2775.]
L-イソロイシン 上流と下流の製品情報(bào)
原材料
準(zhǔn)備製品