DIG-Northern Starter Kit Chemische Eigenschaften,Einsatz,Produktion Methoden

Verwenden

The DIG Northern Starter Kit produces DIG-labeled RNA probes that can be used in conjunction with the supplied chemiluminescent detection reagents for northern blotting techniques. Using linearized DNA as a template, SP6, T3, or T7 RNA Polymerases are used to incorporate DIG-11-UTP into the RNA transcript. After labeling, the DIG-labeled probe is immediately ready for use in hybridization. For convenience, DIG Easy Hyb can be used for hybridization. The DIG Easy Hyb granules are easily reconstituted by adding 64?ml DEPC-treated (RNase-free) water directly to the bottle (once reconstituted, DIG Easy Hyb is stable for 1 month at room temperature). After hybridization, the hybridization solution containing the DIG-labeled RNA probe can be stored at -15 to -25 °C for future re-use (up to 1 year).

Allgemeine Beschreibung

The DIG Northern Starter Kit is designed for the novice DIG system user. It contains the reagents proven to provide successful, reproducible results in northern blots. Additionally, the more convenient forms of standard DIG system products are included (e.g., CDP Star, ready-to-use). The small number of reactions allows the user to gain a firm foundation using the DIG system, then "graduate" to the standard pack sizes. For optimum success, use this kit with the DIG Wash and Block Buffer Set.This kit is used for the generation of single-stranded, DIG-labeled RNA probes and chemiluminescent detection. Labeled probes are synthesized by the in vitro transcription method.

DIG-Northern Starter Kit Upstream-Materialien And Downstream Produkte

Upstream-Materialien

Downstream Produkte

DIG-Northern Starter Kit Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.

Firmenname	Telefon	E-Mail	Land	Produktkatalog	Edge Rate
Sigma-Aldrich	021-61415566 800-8193336	orderCN@merckgroup.com	China	51456	80

---

• DIG-Northern Starter Kit