N-(1-phenylethyl)-2-(pyrrolidin-1-yl)quinazolin-4-aMine Chemische Eigenschaften,Einsatz,Produktion Methoden
Beschreibung
Importazole is a cell-permeable inhibitor of importin-β, which transports cargo molecules bearing a nuclear localization signal into the nucleus. Importazole prevents nuclear import of GFP-tagged nuclear factor of activated T cells (NFAT-GFP) in HEK293 cells (IC
50 = 15 μM), which is reversible upon importazole washout. Importazole does not interfere with transportin-mediated nuclear import or chromosomal maintenance 1 (CRM1)-mediated nuclear export. Importazole inhibits nuclear import of topoisomerase IIβ binding protein 1 (TopBP1) in
Xenopus egg extracts and of NF-κB in multiple myeloma cells. It also induces defects in spindle and microtubule formation
in vitro.
Verwenden
Importazole is an importin-β inhibitor.
Biochem/physiol Actions
Importazole is an inhibitor of importin-β transport receptors. During interphase, the transport receptor importin-β carries cargoes into the nucleus, where RanGTP releases them. Importazole somehow disrupts the importin/RAN interaction. Importazole is selective among other transporters. Compounds are imported into, but not out of, cells.
N-(1-phenylethyl)-2-(pyrrolidin-1-yl)quinazolin-4-aMine Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
