First Strand cDNA Synthesis Kit for RT-PCR (AMV) Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
The First Strand cDNA Synthesis Kit for RT-PCR (AMV) is suitable for:
- Detection of the presence or absence of RNA viruses or other RNA-containing microorganisms (in combination with PCR)
- Quantification of mRNA for monitoring differential expression of a specific mRNA
- First step in the "differential display of mRNA"
- Generation of cDNA libraries with large and full-length inserts
- Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for reverse transcription of RNA into complementary DNA.
Allgemeine Beschreibung
The use of AMV RT for first-strand cDNA synthesis has certain advantages. The higher thermostability of this enzyme compared to M-MuLV RT allows the reaction to be performed at +42°C, giving higher specificity and better resolution of secondary structures compared to a reaction performed at +37°C.
First Strand cDNA Synthesis Kit for RT-PCR (AMV) Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte