ALPHA-KETOGLUTARATE DEHYDROGENASE
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- CAS-Nr.
- 9031-02-1
- Englisch Name:
- ALPHA-KETOGLUTARATE DEHYDROGENASE
- Synonyma:
- α-Ketoglutarate Dehydrogenase;ALPHA-KETOGLUTARATE DEHYDROGENASE;α-ketoglutarate dehydrogenase from porcine heart;multienzyme 2-oxoglutarate dehydrogenase complex;A-ketoglutarate dehydrogenase from*porcine heart;α-Ketoglutarate Dehydrogenase, from porcine heart;alpha-Ketoglutarate Dehydrogenase from porcine heart;MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2
- CBNumber:
- CB0239707
- Summenformel:
- Molgewicht:
- 0
- MOL-Datei:
- Mol file
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ALPHA-KETOGLUTARATE DEHYDROGENASE Eigenschaften
- storage temp.
- -20°C
- Aggregatzustand
- buffered aqueous glycerol solution
- Specific Activity
- 0.1-1.0units/mg protein (Lowry)
Sicherheit
- Risiko- und Sicherheitserkl?rung
- Gefahreninformationscode (GHS)
| Bildanzeige (GHS) |
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| Alarmwort |
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| Gefahrenhinweise |
| Code |
Gefahrenhinweise |
Gefahrenklasse |
Abteilung |
Alarmwort |
Symbol |
P-Code |
| H412 |
Sch?dlich für Wasserorganismen, mit langfristiger Wirkung. |
Langfristig (chronisch) gew?ssergef?hrdend |
Kategorie 3 |
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P273, P501 |
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| Sicherheit |
| P273 |
Freisetzung in die Umwelt vermeiden. |
| P501 |
Inhalt/Beh?lter ... (Entsorgungsvorschriften vom Hersteller anzugeben) zuführen. |
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ALPHA-KETOGLUTARATE DEHYDROGENASE Chemische Eigenschaften,Einsatz,Produktion Methoden
Verwenden
α-Ketoglutarate Dehydrogenase from the porcine heart has been used:
- to study the reversal of nitration by glutathione (GSH) in peroxynitrite-treated cells
- to measure its activity by Spectramax M5 microplate spectrofluorimeter using heart mitochondria
- as a positive control to evaluate its activity in by Spectramax GEMINI EM fluorescence microplate reader using mice neurons
Allgemeine Beschreibung
α-Ketoglutarate dehydrogenase (α-KGDH) is a multienzyme complex localized to the mitochondria. This integrated enzyme is made up of many units of thiamine pyrophosphate-dependent dehydrogenase (E1), dihydrolipoamide dehydrogenase (E3), and dihydrolipoamide succinyl transferase (E2).
Biochem/physiol Actions
α-Ketoglutarate dehydrogenase (α-KGDH) is a key enzyme of bioenergetic processes and a controlling unit of metabolic flux through the Krebs cycle or tricarboxylic acid (TCA) cycle. It catalyzes the oxidative decarboxylation of α-ketoglutarate (KG) to succinyl-CoA by releasing reduced nicotinamide adenine dinucleotide (NADH). It is the rate-limiting reaction of the TCA cycle. This reaction contributes to the electrons of the respiratory chain and requires thiamine pyrophosphate as a cofactor. The reduction of NAD (nicotinamide adenine dinucleotide) is observed to determine its reaction rate. α-KGDH from porcine has an optimum pH range of 6.6–7.4. This enzyme is inhibited by oxidative stress and results in a metabolic deficiency. However, α-KGDH is also known to produce reactive oxygen species (ROS) leading to oxidative stress. Defective or limited levels of α-KGDH cause several neurodegenerative diseases such as Alzheimer′s disease.
ALPHA-KETOGLUTARATE DEHYDROGENASE Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
ALPHA-KETOGLUTARATE DEHYDROGENASE Anbieter Lieferant Produzent Hersteller Vertrieb H?ndler.
Global( 7)Lieferanten
- ALPHA-KETOGLUTARATE DEHYDROGENASE
- A-ketoglutarate dehydrogenase from*porcine heart
- multienzyme 2-oxoglutarate dehydrogenase complex
- α-ketoglutarate dehydrogenase from porcine heart
- MULTIENZYME 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, (1) INCLUDING EC 1.2.4.2
- α-Ketoglutarate Dehydrogenase
- alpha-Ketoglutarate Dehydrogenase from porcine heart
- α-Ketoglutarate Dehydrogenase, from porcine heart
- 9031-02-1
- BioChemical
- General Metabolic Enzymes I-Z
- General Metabolic Enzymes
- Metabolomics
